ATP5PB Antibody (OAGA04870)

Catalog No: OAGA04870

Size:100 ul

Sample (30 ug of whole cell lysate)
A: IMR32
12% SDS PAGE
diluted at 1:5000

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using ATP synthase B1 antibody at 1:250 dilution.

ATP synthase B1 antibody [N1C3] detects ATP synthase B1 protein at mitochondria by immunofluorescent analysis.
Sample: SKNSH cells were fixed in 2% paraformaldehyde/culture medium at 37℃ for 30 min.
Green: ATP synthase B1 protein stained by ATP synthase B1 antibody [N1C3] diluted at 1:500.
Blue: Hoechst 33342 staining.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, IHC-P, WB
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human ATP synthase B1. The exact sequence is proprietary.
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 0.51 mg/ml
• Preservative: 0.01% Thimerosal
• Application Info: Suggested starting dilutions are as follows: ICC/IF: 1:100-1:1000, IHC-P: 1:100-1:1000, WB: 1:1000-1:10000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: ATP5PB
• Gene Full Name: ATP synthase peripheral stalk-membrane subunit b
• Alias Symbols: PIG47, ATP5F1
• NCBI Gene Id: 515
• Protein Name: ATP synthase F(0) complex subunit B1, mitochondrial
• Description of Target: This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the b subunit of the proton channel.
• Uniprot ID: P24539
• Protein Accession #: NP_001679.2
• Nucleotide Accession #: NM_001688.4