ATP5J ELISA Kit (Rat) (OKCD01271)

Catalog No: OKCD01271 (Formerly GWB-KBBNN4)

Size:96WELLS

Rat Atp5j ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat|Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	78-93%	95-104%	87-101%	81-93%
  EDTA plasma(n=5)	82-90%	96-105%	93-101%	80-101%
  heparin plasma(n=5)	80-101%	79-104%	82-91%	79-96%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Coupling Factor 6 (CF6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Coupling Factor 6 (CF6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Coupling Factor 6 (CF6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Coupling Factor 6 (CF6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.781-50ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	84-92	88
  EDTA plasma(n=5)	90-104	99
  heparin plasma(n=5)	91-99	95

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Coupling Factor 6 (CF6) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Coupling Factor 6 (CF6) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-Atp5j Microplate: 96 Wells (12 x 8 Well Strips)
  • Atp5j Lyophilized Standard: 2 x 50 ng
  • 100X Biotinylated Atp5j Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids.
• Sensitivity: < 0.29 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Coupling Factor 6 (CF6). No significant cross-reactivity or interference between Coupling Factor 6 (CF6) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: Atp5pf
• Gene Full Name: ATP synthase peripheral stalk subunit F6
• Alias Symbols: ATP synthase, H+ transporting, mitochondrial F0 complex, subunit F6;ATP synthase, H+ transporting, mitochondrial Fo complex, subunit F6;ATP synthase-coupling factor 6, mitochondrial;Atp5j;ATPase subunit F6;coupling factor 6.
• NCBI Gene Id: 94271
• Protein Name: ATP synthase-coupling factor 6, mitochondrial
• Description of Target: Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements. Also involved in the restoration of oligomycin-sensitive ATPase activity to depleted F1-F0 complexes (By similarity).
• Uniprot ID: P21571
• Protein Accession #: NP_001003696
• Nucleotide Accession #: NM_001003696.1
• Protein Size (# AA): 108