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Catalog No: OKCD08926
Size:96WELLS
Price: $850.00
SKU
OKCD08926
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Datasheets/ManualsPrintable datasheet for ATP5F1B ELISA Kit (Rat) (OKCD08926)
Product Info
Predicted Species ReactivityRat|Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ATPase, H+ Transporting, Mitochondrial F1 Complex Beta Polypeptide (ATP5b). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to ATPase, H+ Transporting, Mitochondrial F1 Complex Beta Polypeptide (ATP5b). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ATPase, H+ Transporting, Mitochondrial F1 Complex Beta Polypeptide (ATP5b), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of ATPase, H+ Transporting, Mitochondrial F1 Complex Beta Polypeptide (ATP5b) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range3.12-200ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ATPase, H+ Transporting, Mitochondrial F1 Complex Beta Polypeptide (ATP5b) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ATPase, H+ Transporting, Mitochondrial F1 Complex Beta Polypeptide (ATP5b) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
ATP5F1B Microplate96 Wells (12 x 8 Well strips)
ATP5F1B Lyophilized Standard2
100X ATP5F1B HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 2 mL
Stop Solution1 x 20 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity< 1.21ng/mL
Gene SymbolAtp5f1b
Gene Full NameATP synthase F1 subunit beta
Alias SymbolsATP synthase F1 subunit beta;ATP synthase subunit beta, mitochondrial;ATP synthase, H+ transporting mitochondrial F1 complex, beta subunit;ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide;Atp5b;mitochondrial ATP synthase beta subunit.
NCBI Gene Id171374
Protein NameATP synthase subunit beta, mitochondrial
Description of TargetMitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.
Uniprot IDP10719
Protein Accession #NP_599191.1
Nucleotide Accession #NM_134364.1
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