ATP5F1 Antibody (OACA07366)

Catalog No: OACA07366

Immunohistochemistry of paraffin-embedded human colon cancer at dilution 1:100

All lanes: ATP synthase F(0) complex subunit B1, mitochondrial antibody at 4ug/ml
Lane 1: mouse heart tissue
Lane 2: mouse skeletal muscle tissue
Secondary
Goat polyclonal to rabbit at 1/1,0000 dilution
Predicted band size: 29kDa
Observed band size: 29kDa

All lanes: ATP synthase F(0) complex subunit B1, mitochondrial antibody at 4ug/ml
Lane 1: mouse heart tissue
Lane 2: mouse skeletal muscle tissue
Secondary
Goat polyclonal to rabbit at 1/1,0000 dilution
Predicted band size: 29kDa
Observed band size: 29kDa

Immunohistochemistry of paraffin-embedded human colon cancer at dilution 1:100

Product Info

• Predicted Species Reactivity: Human|Mouse
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay|Immunohistochemistry|Western blot
• Reconstitution and Storage: -20°C or -80°C
• Immunogen: Recombinant Human ATP synthase F(0) complex subunit B1, mitochondrial protein (1-245AA)
• Purification: Affinity Purified
• Preservative: 0.02% sodium azide
• Formulation: PBS with, 50% glycerol, pH7.3.
• Reference: [1]"An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.J. Proteomics 96:253-262(2013). [2]"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.BMC Syst. Biol. 5:17-17(2010). [3]"Lysine acetylation targets protein complexes and co-regulates major cellular functions."Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M.Science 325:834-840(2008).

Target Info

• Gene Symbol: ATP5PB
• Gene Full Name: ATP synthase peripheral stalk-membrane subunit b
• Alias Symbols: ATP synthase B chain, mitochondrial;ATP synthase F(0) complex subunit B1, mitochondrial;ATP synthase proton-transporting mitochondrial F(0) complex subunit B1;ATP synthase subunit b;ATP synthase subunit b, mitochondrial;ATP synthase, H+ transporting, mitochondrial F0 complex, subunit b;ATP synthase, H+ transporting, mitochondrial F0 complex, subunit B1;ATP synthase, H+ transporting, mitochondrial Fo complex subunit B1;ATP5F1;ATPase subunit b;cell proliferation-inducing protein 47;H+-ATP synthase subunit b;PIG47.
• NCBI Gene Id: 515
• Protein Name: ATP synthase F(0) complex subunit B1, mitochondrial
• Description of Target: Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements.
• Uniprot ID: P24539
• Protein Accession #: NP_001679
• Nucleotide Accession #: NM_001688