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Catalog No: OKEH05591
Size:96T
Price: $625.00
SKU
OKEH05591
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology Atp5b ELISA Kit (Mouse) (OKEH05591) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Atp5b has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Atp5b is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Atp5b captured in the well.
ELISA Kit Range31.2-2000pg/mL
ELISA Kit RecoveryMean recovery when spiking into Serum and Plasma = 85.9%
ELISA Kit ReproducibilityThree samples concentrations were measured in replicate within an assay plate and across replicate assays to assess Intra- and Mean Inter-Assay Precision.
Mean Intra-Assay Precision - <6.8%, n = 20
Mean Inter-Assay Precision - <9.4%, n = 20
ELISA Kit Component
ComponentAmount
ATP5B Microplate96 Wells (12 x 8 Well strips)
ATP5B Lyophilized Standard2
100X Biotinylated ATP5B Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma and other biological fluids
Sensitivity15.6 pg/mL
SpecificityNatural and recombinant Mouse ATP synthase subunit beta, mitochondrial
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolAtp5b
Gene Full NameATP synthase, H+ transporting mitochondrial F1 complex, beta subunit
Alias SymbolsATP synthase F1 subunit beta;ATP synthase subunit beta, mitochondrial;ATP synthase, H+ transporting mitochondrial F1 complex, alpha subunit;Atp5f1b;mitochondrial ATP synthase, H+ transporting F1 complex beta subunit.
NCBI Gene Id11947
Protein NameP56480
Description of TargetATP synthase subunit beta, mitochondrial
Uniprot IDhttps://www.uniprot.org/uniprot/P56480
Protein Accession #NP_058054.2
Nucleotide Accession #NM_016774.3
Protein Size (# AA)529
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