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Catalog No: OKEH02247
Price: $625.00
Availability: Domestic: within 2-3 weeks delivery | International: 2-3 weeks
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ATP5B ELISA Kit (Human) : 96 Wells (OKEH02247)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology ATP5B ELISA Kit (Human) (OKEH02247) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ATP5B has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ATP5B is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ATP5B captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 88%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.2% (n = 20)
Mean Inter-assay CV%: < 8.3% (n = 20)
ELISA Kit Component
ATP5B Microplate96 Wells (12 x 8 Well strips)
ATP5B Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated PGK1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.084 ng/mL
SpecificityNatural and recombinant Human ATP synthase subunit beta, mitochondrial
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolATP5F1B
Gene Full NameATP synthase F1 subunit beta
Alias SymbolsATP synthase F1 subunit beta;ATP synthase subunit beta, mitochondrial;ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide;ATP5B;ATPMB;ATPSB;epididymis secretory protein Li 271;HEL-S-271;mitochondrial ATP synthase beta subunit;mitochondrial ATP synthetase, beta subunit.
NCBI Gene Id506
Protein NameP06576
Description of TargetATP synthase subunit beta, mitochondrial
Uniprot ID
Protein Accession #NP_001677.2
Nucleotide Accession #NM_001686.3
Protein Size (# AA)529
Molecular Weight57 kDa
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