ATP5B ELISA Kit (Human) : 96 Wells (OKEH02247)

Catalog No: OKEH02247

Size:96T

Human ATP5B ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology ATP5B ELISA Kit (Human) (OKEH02247) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ATP5B has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ATP5B is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ATP5B captured in well.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 88%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 5.2% (n = 20)
  Mean Inter-assay CV%: < 8.3% (n = 20)
• ELISA Kit Component:
  • ATP5B Microplate: 96 Wells (12 x 8 Well strips)
  • ATP5B Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X Biotinylated PGK1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.084 ng/mL
• Specificity: Natural and recombinant Human ATP synthase subunit beta, mitochondrial
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: ATP5F1B
• Gene Full Name: ATP synthase F1 subunit beta
• Alias Symbols: ATP synthase F1 subunit beta;ATP synthase subunit beta, mitochondrial;ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide;ATP5B;ATPMB;ATPSB;epididymis secretory protein Li 271;HEL-S-271;mitochondrial ATP synthase beta subunit;mitochondrial ATP synthetase, beta subunit.
• NCBI Gene Id: 506
• Protein Name: P06576
• Description of Target: ATP synthase subunit beta, mitochondrial
• Uniprot ID: https://www.uniprot.org/uniprot/P06576
• Protein Accession #: NP_001677.2
• Nucleotide Accession #: NM_001686.3
• Protein Size (# AA): 529
• Molecular Weight: 57 kDa