ATP5B Recombinant Antibody [5F10] (OACA12391)

Catalog No: OACA12391

Size:100UL

Western Blot
Positive WB detected in: 293T whole cell lysate, HT29 whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, 293 whole cell lysate, Rat Heart tissue, Mouse Heart tissue
All lanes: ATP5F1B antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 57 kDa
Observed band size: 57 kDa

IHC image of OACA12391 diluted at 1:100 and staining in paraffin-embedded human heart tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

IHC image of OACA12391 diluted at 1:100 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid
• Clonality: Recombinant Monoclonal
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay,
  Immunohistochemistry,
  Western blot
• Reconstitution and Storage: -20°C or -80°C
• Immunogen: A synthesized peptide derived from human ATPB
• Purification: Affinity Purified

Target Info

• Gene Symbol: ATP5F1B
• Gene Full Name: ATP synthase F1 subunit beta
• Alias Symbols: ATP synthase F1 subunit beta;ATP synthase subunit beta, mitochondrial;ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide;ATP5B;ATPMB;ATPSB;epididymis secretory protein Li 271;HEL-S-271;mitochondrial ATP synthase beta subunit;mitochondrial ATP synthetase, beta subunit.
• NCBI Gene Id: 506
• Protein Name: ATP synthase subunit beta, mitochondrial
• Description of Target: Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.
• Uniprot ID: P06576