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Datasheets/Manuals | Printable datasheet for anti-ATP5B (ARP89713_P050) antibody |
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Predicted Species Reactivity | Mouse |
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Product Format | Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Clonality | Polyclonal |
Host | Rabbit |
Application | WB |
Reconstitution and Storage | For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
Immunogen | The immunogen is a synthetic peptide directed towards the middle region of mouse ATP5B |
Purification | Affinity purified |
Peptide Sequence | Synthetic peptide located within the following region: GGYSVFAGVGERTREGNDLYHEMIESGVINLKDATSKVALVYGQMNEPPG |
Concentration | 0.5 mg/ml |
Blocking Peptide | For anti-ATP5B (ARP89713_P050) antibody is Catalog # AAP89713 |
Gene Symbol | ATP5B |
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Gene Full Name | ATP synthase, H+ transporting mitochondrial F1 complex, beta subunit |
Alias Symbols | Atp5f1b |
NCBI Gene Id | 11947 |
Protein Name | ATP synthase subunit beta, mitochondrial |
Description of Target | Mitochondrial membrane ATP synthase (F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F1 - containing the extramembraneous catalytic core, and F0 - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F1. Rotation of the central stalk against the surrounding alpha3beta3 subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. |
Uniprot ID | P56480 |
Protein Accession # | NP_058054.2 |
Nucleotide Accession # | NM_016774.3 |
Protein Size (# AA) | 529 |
Molecular Weight | 56 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "ATP5B Antibody - middlel region (ARP89713_P050)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Mouse".
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How long will it take to receive "ATP5B Antibody - middlel region (ARP89713_P050)"?
This item is available "Domestic: within 24 hours delivery | International: 3-5 days".
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What buffer format is "ATP5B Antibody - middlel region (ARP89713_P050)" provided in?
This item is provided in "Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "ATP5B Antibody - middlel region (ARP89713_P050)"?
This target may also be called "Atp5f1b" in publications.
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What is the shipping cost for "ATP5B Antibody - middlel region (ARP89713_P050)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "ATP5B Antibody - middlel region (ARP89713_P050)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "ATP5B Antibody - middlel region (ARP89713_P050)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "56 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "ATP5B Antibody - middlel region (ARP89713_P050)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "ATP5B"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "ATP5B"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "ATP5B"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "ATP5B"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "ATP5B"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "ATP5B"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.