ATP2B2 ELISA Kit (Mouse) (OKCD02393)

Catalog No: OKCD02393

Size:96WELLS

Mouse ATP2B2 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ATPase, Ca++ Transporting, Plasma Membrane 2 (ATP2B2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to ATPase, Ca++ Transporting, Plasma Membrane 2 (ATP2B2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ATPase, Ca++ Transporting, Plasma Membrane 2 (ATP2B2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of ATPase, Ca++ Transporting, Plasma Membrane 2 (ATP2B2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ATPase, Ca++ Transporting, Plasma Membrane 2 (ATP2B2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ATPase, Ca++ Transporting, Plasma Membrane 2 (ATP2B2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-ATP2B2 Microplate: 96 Wells (12 x 8 Well strips)
  • ATP2B2 Lyophilized Standard: 2
  • 100X Biotinylated ATP2B2 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: 0.058 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of ATPase, Ca++ Transporting, Plasma Membrane 2 (ATP2B2). No significant cross-reactivity or interference between ATPase, Ca++ Transporting, Plasma Membrane 2 (ATP2B2) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Atp2b2
• Gene Full Name: ATPase, Ca++ transporting, plasma membrane 2
• Alias Symbols: D6Abb2;D6Abb2e;deaf waddler;dfw;Gena3;Gena300;jo;jog;plasma membrane calcium ATPase;Plasma membrane calcium ATPase isoform 2;plasma membrane calcium pump;Plasma membrane calcium pump isoform 2;plasma membrane calcium-transporting ATPase 2;PMC;PMCA2;Tmy;wm;wms;wri;wriggle.
• NCBI Gene Id: 11941
• Protein Name: Plasma membrane calcium-transporting ATPase 2
• Description of Target: This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium out of the cell. Plays a role in maintaining balance and hearing.
• Uniprot ID: Q9R0K7
• Protein Accession #: NP_001031761.1
• Nucleotide Accession #: NM_001036684.3
• Protein Size (# AA): 1198