ATP2A3 ELISA Kit (Rat) (OKEH03732)

Catalog No: OKEH03732

Size:96T

Rat ATP2A3 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Rat, Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology ATP2A3 ELISA Kit (Rat) (OKEH03732) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Atp2a3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Atp2a3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Atp2a3 captured in well.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Component:
  • Atp2a3 Microplate: 96 Wells (12 x 8 Well strips)
  • Atp2a3 Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X Biotinylated Atp2a3 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 10 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.168 ng/mL
• Specificity: Natural and recombinant Rat Sarcoplasmic/endoplasmic reticulum calcium ATPase 3
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Atp2a3
• Gene Full Name: ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 3
• Alias Symbols: ATPase, Ca++ transporting, ubiquitous;calcium pump 3;sarcoplasmic/endoplasmic reticulum calcium ATPase 3;SERCA3;SR Ca(2+)-ATPase 3.
• NCBI Gene Id: 25391
• Protein Name: P18596
• Description of Target: Sarcoplasmic/endoplasmic reticulum calcium ATPase 3
• Uniprot ID: https://www.uniprot.org/uniprot/P18596
• Protein Accession #: NP_037046.1
• Nucleotide Accession #: NM_012914.1
• Protein Size (# AA): 1061
• Molecular Weight: 116 kDa