ATP2A1 ELISA Kit (Rat) (OKEH06153)

Catalog No: OKEH06153

Size:96 Wells

Rat ATP2A1 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Rat
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~3 Hours
• ELISA Kit Principle: Aviva Systems Biology Atp2a1 ELISA Kit (Rat) (OKEH06153) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Atp2a1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Atp2a1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Atp2a1 captured in well.
• ELISA Kit Range: 0.312 - 20ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into Serum and Plasma = 110%
• ELISA Kit Reproducibility: Three samples concentrations were measured in replicate within an assay plate and across replicate assays to assess Intra- and Mean Inter-Assay Precision.
  Mean Intra-Assay Precision - <4.9%, n = 20
  Mean Inter-Assay Precision - <8.1%, n = 20
• ELISA Kit Component:
  • ATP2A1 Microplate: 96 Wells (12 x 8 Well strips)
  • ATP2A1 Lyophilized Standard: 2
  • 100X Biotinylated ATP2A1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 0.156 ng/mL
• Specificity: Natural and recombinant Rat Sarcoplasmic/endoplasmic reticulum calcium ATPase 1
• Assay Info: Assay Methodology: Quantitative Competitive ELISA

Target Info

• Gene Symbol: ATP2A1
• Gene Full Name: ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 1
• Alias Symbols: Serca1
• NCBI Gene Id: 116601
• Protein Name: Sarcoplasmic/endoplasmic reticulum calcium ATPase 1
• Description of Target: Key regulator of striated muscle performance by acting as the major Ca2+ ATPase responsible for the reuptake of cytosolic Ca2+ into the sarcoplasmic reticulum. Catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction.
• Uniprot ID: Q64578
• Protein Accession #: NP_478120.1
• Nucleotide Accession #: NM_058213.1
• Protein Size (# AA): 994