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ATP2A1 Antibody (OAAD00121)

100 ug
$255.00
In Stock
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Gene Symbol:
ATP2A1
NCBI Gene Id:
487
Official Gene Full Name:
Sarcoplasmic/endoplasmic reticulum calcium ATPase 1
Protein Name:
Sarcoplasmic/endoplasmic reticulum calcium ATPase 1
Swissprot Id:
O14983
Protein Accession #:
NP_001273004.1; NP_004311.1; NP_775293.1
Nucleotide Accession #:
NM_001286075.1; NM_004320.4; NM_173201.3
Replacement Item:
This antibody may replace item sc-12493 from Santa Cruz Biotechnology.
Description of Target:
The Ca(2+)-ATPases are a family of plasma membrane pumps encoded by at least 4 genes: ATP2B1 on chromosome 12q21; ATP2B2; ATP2B3 on Xq28; and ATP2B4 on 1q25. The proteins share 84% amino acid sequence identity and 76% nucleic acid sequence homology. The function of calcium-transporting ATPase found in different membranes is to lower cytoplasmic Ca(2+) concentration by pumping Ca(2+) to luminal or extracellular spaces. Mutations in a plasma membrane Ca(2+)-ATPase gene cause deafness in deafwaddler mice.
Host:
Mouse
Clonality:
Monoclonal
Purification:
Purified by the goat anti-mouse IgG Affinity chromatography.
Application:
WB, IHC, IP
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express ATP2A1.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express ATP2A1.
Immunogen:
Human erythrocyte Ca2+-ATPase.
Predicted Species Reactivity:
Human, Rat, Rabbit Chicken
Reconstitution and Storage:
At -20C for one year. After reconstitution, at 4C for one month. It can also be aliquotted and stored frozen at -20C for longer time.
Datasheets/Manuals:
Printable datasheet for anti-ATP2A1 (OAAD00121) antibody
Specificity:
Human, rat, rabbit. chicken.
No cross reactivity with other proteins.
Formulation:
Lyophilized from 1.2% sodium acetate, with 2mg BSA and 0.01mg NaN3 as preservative.
Clone:
CAP-6
Isotype:
IgG2a
Application Info:
Western blot At 4ug/ml with the appropriate system to detect Ca2+ ATPase in cells and tissues.
Immunohistochemistry(P) At 8ug/ml to detect ATPase in formalin fixed and paraffin embedded tissues.
Other applications have not been tested.
Optimal dilutions should be determined by end user.
Storage Buffer:
1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100ug/ml.
Target Reference:
Street, V. A.; McKee-Johnson, J. W.; Fonseca, R. C.; Tempel, B. L.; Noben-Trauth, K. : Mutations in a plasma membrane Ca(2+)-ATPase gene cause deafness in deafwaddler mice. Nature Genet. 19: 390-394, 1998.

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