ATP1B3 ELISA Kit (Rat) (OKCD06716)

Catalog No: OKCD06716

Size:96WELLS

Product Info

• Predicted Species Reactivity: Rat|Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sodium/potassium Transporting ATPase Subunit Beta-3 (ATP1b3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Sodium/potassium Transporting ATPase Subunit Beta-3 (ATP1b3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sodium/potassium Transporting ATPase Subunit Beta-3 (ATP1b3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Sodium/potassium Transporting ATPase Subunit Beta-3 (ATP1b3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Sodium/potassium Transporting ATPase Subunit Beta-3 (ATP1b3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Sodium/potassium Transporting ATPase Subunit Beta-3 (ATP1b3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • ATP1B3 Microplate: 96 Wells (12 x 8 Well strips)
  • ATP1B3 Lyophilized Standard: 2
  • 100X ATP1B3 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 2 mL
  • Stop Solution: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates and other biological fluids.
• Sensitivity: < 0.071ng/mL

Target Info

• Gene Symbol: Atp1b3
• Gene Full Name: ATPase Na+/K+ transporting subunit beta 3
• Alias Symbols: ATPase, Na+/K+ transporting, beta 3 polypeptide;ATPase, Na+K+ transporting, beta polypeptide 3;ATPB-3;Na+/K+ -ATPase beta 3 subunit;NKAB3S;sodium/potassium-dependent ATPase subunit beta-3;sodium/potassium-transporting ATPase subunit beta-3.
• NCBI Gene Id: 25390
• Protein Name: Sodium/potassium-transporting ATPase subunit beta-3
• Description of Target: This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The exact function of the beta-3 subunit is not known.
• Uniprot ID: Q63377
• Protein Accession #: NP_037045.1
• Nucleotide Accession #: NM_012913.1