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Catalog No: OKEH05889
Price: $525.00
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology Atoh1 ELISA Kit (Mouse) (OKEH05889) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Atoh1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Atoh1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Atoh1 captured in well.
ELISA Kit Range0.78-50ng/mL
ELISA Kit RecoveryMean recovery when spiking into Serum and Plasma = 105.1%
ELISA Kit ReproducibilityThree samples concentrations were measured in replicate within an assay plate and across replicate assays to assess Intra- and Mean Inter-Assay Precision.
Mean Intra-Assay Precision - <6.9%, n = 20
Mean Inter-Assay Precision - <8.8%, n = 20
ELISA Kit Component
ATOH1 Microplate96 Wells (12 x 8 Well strips)
ATOH1 Lyophilized Standard2
100X Biotinylated ATOH1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma and other biological fluids
Sensitivity0.409 ng/mL
SpecificityNatural and recombinant Mouse Protein atonal homolog 1
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolAtoh1
Gene Full Nameatonal bHLH transcription factor 1
Alias Symbolsatonal homolog 1;bHLHa;bHLHa14;Hath;Hath1;helix-loop-helix protein mATH-1;Math;Math1;MATH-1;protein atonal homolog 1.
NCBI Gene Id11921
Protein NameP48985
Description of TargetProtein atonal homolog 1
Uniprot ID
Protein Accession #NP_031526.1
Nucleotide Accession #NM_007500.4
Protein Size (# AA)351
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