ATG5 ELISA Kit (Bovine) (OKEH08111)

Catalog No: OKEH08111

Size:96T

Product Info

• Predicted Species Reactivity: Bos taurus, Bovine
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology ATG5 ELISA Kit (Bovine) (OKEH08111) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ATG5 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ATG5 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ATG5 captured in well.
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 115% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=2.5% (n = 20)
  Mean Inter-assay CV%: <=10.7% (n = 20)
• ELISA Kit Component:
  • ATG5 Microplate: 96 Wells (12 x 8 Well strips)
  • ATG5 Lyophilized Standard: 2
  • 100X Biotinylated ATG5 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: May play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. Plays a crucial role in IFN-gamma-induced autophagic cell death by interacting with FADD.
  Subcellular Location: Cytoplasm Preautophagosomal structure membrane Peripheral membrane protein The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. Localizes also to discrete punctae along the ciliary axoneme and to the base of the ciliary axoneme.
  Unigene: Bt.27505
  SMR: Q3MQ24
  String: 9913.ENSBTAP00000007100
  KEGG: bta:532686
  Pfam: PF04106
• Reconstitution and Storage: Store as indicated in product manual.
• Specificity: Natural and recombinant Bovine Autophagy protein 5
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: ATG5
• Gene Full Name: autophagy related 5
• Alias Symbols: ATG5 autophagy related 5 homolog;autophagy protein 5.
• NCBI Gene Id: 532686
• Protein Name: Q3MQ24
• Description of Target: Autophagy protein 5
• Uniprot ID: https://www.uniprot.org/uniprot/Q3MQ24
• Protein Accession #: NP_001029751.2
• Nucleotide Accession #: NM_001034579.2