ATG4B Antibody (OAAF02774)

Catalog No: OAAF02774 (Formerly GWB-ASD968)

Size:100 ug

Western blot analysis of extracts from Jurkat cells, using ATG4B Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human skeletal muscle tissue, using ATG4B Antibody. The picture on the right is treated with the synthesized peptide.

Product Info

• Predicted Species Reactivity: Human|Mouse
• Product Format: Liquid
• Clonality: Polyclonal
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot
• Reconstitution and Storage: -20°C
• Immunogen: The antiserum was produced against synthesized peptide derived from human ATG4B.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Specificity: ATG4B antibody detects endogenous levels of total ATG4B protein.
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Dilution: The optimal working dilution should be determined by the end user.
• Application Info: WB: 1:500~1:3000; IHC: 1:50~1:100; ELISA: 1:20000
• Reference: Kabeya Y., J. Cell Sci. 117:2805-2812(2004).
  Marino G., J. Biol. Chem. 278:3671-3678(2003).
  Ota T.,Nat. Genet. 36:40-45(2004).
• Publications:

  NKCT1 (purified Naja kaouthia protein toxin) conjugated gold nanoparticles induced Akt/mTOR inactivation mediated autophagic and caspase 3 activated apoptotic cell death in leukemic cell. Toxicon. 121, 86-97 (2016). 27527270

Target Info

• Gene Symbol: ATG4B
• Gene Full Name: autophagy related 4B cysteine peptidase
• Alias Symbols: APG4 autophagy 4 homolog B;APG4B;ATG4 autophagy related 4 homolog B;AUTL1;AUT-like 1 cysteine endopeptidase;autophagin-1;autophagy-related cysteine endopeptidase 1;autophagy-related protein 4 homolog B;cysteine protease ATG4B;hAPG4B.
• NCBI Gene Id: 23192
• Protein Name: Cysteine protease ATG4B
• Description of Target: Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3, GABARAPL1, GABARAPL2 and GABARAP, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms.
• Uniprot ID: Q9Y4P1
• Protein Accession #: NP_037457.3
• Molecular Weight: 44 kDa