ATG4B Antibody - N-terminal region (ARP57862_P050)

Catalog No: ARP57862_P050

WB Suggested Anti-ATG4B Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:1562500
Positive Control: Human Placenta

WB Suggested Anti-ATG4B antibody Titration: 1 ug/mL
Sample Type: Human heart

Rabbit Anti-ATG4B Antibody
Catalog Number: ARP57862_P050
Formalin Fixed Paraffin Embedded Tissue: Human Adult heart
Observed Staining: Cytoplasmic
Primary Antibody Concentration: 1:100
Secondary Antibody: Donkey anti-Rabbit-Cy2/3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 – 2.0 sec
Protocol located in Reviews and Data.

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Rabbit
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Clonality: Polyclonal
• Host: Rabbit
• Application: WB, IHC
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human ATG4B
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 92%; Dog: 93%; Guinea Pig: 100%; Human: 100%; Mouse: 93%; Rabbit: 85%; Rat: 100%
• Peptide Sequence: Synthetic peptide located within the following region: WGCMLRCGQMIFAQALVCRHLGRDWRWTQRKRQPDSYFSVLNAFIDRKDS
• Concentration: 0.5 mg/ml
• Blocking Peptide: For anti-ATG4B (ARP57862_P050) antibody is Catalog # AAP57862 (Previous Catalog # AAPP32215)
• Reference: Olsen,J.V., (2006) Cell 127 (3), 635-648

Target Info

• Gene Symbol: ATG4B
• Gene Full Name: ATG4 autophagy related 4 homolog B (S. cerevisiae)
• Alias Symbols: APG4B, AUTL1
• NCBI Gene Id: 23192
• Protein Name: Cysteine protease ATG4B
• Description of Target: Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. ATG4B is a member of the autophagin protein family, it is also designated as a member of the C-54 family of cysteine proteases. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
• Uniprot ID: Q9Y4P1
• Protein Accession #: NP_037457
• Nucleotide Accession #: NM_013325
• Protein Size (# AA): 393
• Molecular Weight: 44kDa
• Protein Interactions: SUMO2; MAP1LC3B; MAP1LC3A; BAG3; ATG8; YWHAB; GABARAPL1; SQSTM1; UBC; GABARAPL2; ATG10; ATG3; AMBRA1; GABARAP; CAMKK2; ATG12; ULK1; STK3; KRT4; ANXA1; FBXW11; LINC00341;