ATG3 Antibody - C-terminal region (OAAB13530)

Catalog No: OAAB13530

Size:400ul

APG3L Antibody (I98) (Cat. # OAAB13530) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the APG3L antibody detected the APG3L protein (arrow).

Western blot analysis of APG3L (arrow) using rabbit polyclonal APG3L Antibody (I98) (Cat. # OAAB13530). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the APG3L gene.

ATG3 is supported by BioGPS gene expression data to be expressed in HEK293, HL60

Product Info

• Predicted Species Reactivity: Human
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB
• Additional Information: Function: E2 conjugating enzyme required for the cytoplasm to vacuole transport (Cvt), autophagy, and mitochondrial homeostasis. Responsible for the E2-like covalent binding of phosphatidylethanolamine to the C-terminal Gly of ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A). The ATG12- ATG5 conjugate plays a role of an E3 and promotes the transfer of ATG8-like proteins from ATG3 to phosphatidylethanolamine (PE). This step is required for the membrane association of ATG8-like proteins. The formation of the ATG8-phosphatidylethanolamine conjugates is essential for autophagy and for the cytoplasm to vacuole transport (Cvt). Preferred substrate is MAP1LC3A. Also acts as an autocatalytic E2-like enzyme, catalyzing the conjugation of ATG12 to itself, ATG12 conjugation to ATG3 playing a role in mitochondrial homeostasis but not in autophagy. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3. Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway.
  Cellular Location: Cytoplasm.
  Tissue Location: Widely expressed, with a highest expression in heart, skeletal muscle, kidney, liver and placenta
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This ATG3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 83-112 amino acids from the C-terminal region of human ATG3.
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Predicted Homology Based on Immunogen Sequence: Cow; Human; Mouse; Rat
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Sample Type Confirmation:

  ATG3 is supported by BioGPS gene expression data to be expressed in HEK293, HL60

• Intended Use: ATG3 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
• Application Info: WB: 1:1000

Target Info

• Gene Symbol: ATG3
• Alias Symbols: APG3, APG3L, PC3-96, APG3-LIKE
• NCBI Gene Id: 64422
• Protein Name: Ubiquitin-like-conjugating enzyme ATG3
• Description of Target: Purified Rabbit Polyclonal Antibody (Pab)
• Uniprot ID: Q9NT62
• Protein Accession #: NP_071933.2
• Molecular Weight: 35864 Da