ATG16L Antibody (OAAB13675)

Catalog No: OAAB13675

Size:400ul

Cos7, HEK293, MEF, and Hela cells, left to right, respectively. Data courtesy of Drs. Jiefei Geng and Dan Klionsky, University of Michigan.

APG16L Antibody (Cat. #OAAB13675) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the APG16L antibody detected the APG16L protein (arrow).

Western blot of APG16L (L92) Pab (Cat. #OAAB13675) in mouse brain tissue lysate.

Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with Autophagy APG16L antibody (L176), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Fluorescent image of U251 cells stained with ATG16L antibody. U251 cells were treated with Chloroquine (50 ?M, 16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with OAAB13675 ATG16L primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ?g/ml, 5 min). ATG16L immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000085978).

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: IF, WB, IHC
• Additional Information: Precautions: ATG16L Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
  Function: Plays an essential role in autophagy (By similarity); interacts with ATG12-ATG5 to mediate the conjugation of phosphatidylethanolamine (PE) to LC3 (MAP1LC3A, MAP1LC3B or MAP1LC3C), to produce a membrane-bound activated form of LC3 named LC3-II. Thereby, controls the elongation of the nascent autophagosomal membrane.
  Cellular Location: Cytoplasm (By similarity). Preautophagosomal structure membrane; Peripheral membrane protein (By similarity) Note=Localized to preautophagosomal structure (PAS) where it is involved in the membrane targeting of ATG5 (By similarity) Localizes also to discrete punctae along the ciliary axoneme (By similarity)
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This ATG16L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 161-190 amino acids from human ATG16L.
• Predicted Homology Based on Immunogen Sequence: Human; Mouse
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Sample Type Confirmation:

  ATG16L1 is strongly supported by BioGPS gene expression data to be expressed in NCI-H460

• Application Info: WB: 1:1000
  IF: 1:25
  IHC-P: 1:10~50

Target Info

• Gene Symbol: ATG16L1
• Alias Symbols: IBD10, WDR30, APG16L, ATG16A, ATG16L
• NCBI Gene Id: 55054
• Protein Name: Autophagy-related protein 16-1
• Description of Target: Purified Rabbit Polyclonal Antibody (Pab)
• Uniprot ID: Q676U5
• Protein Accession #: NP_001177195.1
• Molecular Weight: 68265 Da