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Datasheets/Manuals | Printable datasheet for atg16 Antibody (OAPB00616) (OAPB00616) |
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Predicted Species Reactivity | Human|Mouse|Rat |
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Product Format | Liquid. ATG16 Antibody is supplied in PBS containing 0.02% sodium azide. |
Clonality | Polyclonal |
Host | Rabbit |
Conjugation | Unconjugated |
Application | Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Western blot |
:: | Buffer: Antibody is supplied in PBS containing 0.02% sodium azide. |
Reconstitution and Storage | ATG16 antibody can be stored at 4C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Immunogen | ATG16 antibody was raised against a 18 amino acid synthetic peptide from near the center of human ATG16. The immunogen is located within amino acids 190 - 240 of ATG16. |
Purification | Affinity Purified |
Predicted Homology Based on Immunogen Sequence | Human; Mouse; Rat |
Concentration | 1 mg/ml |
Blocking Peptide | For anti-atg16 (OAPB00616) antibody is Catalog # OEPB02758 |
Application Info | ATG16 antibody can be used for the detection of ATG16 by Western blot at 0.25 - 0.5 ug/ml. (Optimal dilution should be determined by user) |
Other Applications Image 1 Data | Immunocytochemistry of ATG16 in HeLa cells with ATG16 antibody at 2 ug/mL. |
Reference | Gozuacik D and Kimchi A. Autophagy as a cell death and tumor suppressor mechanism. Oncogene.2004; 23:2891-906.;Kisen GO, Tessitore L, Costelli P, et al. Reduced autophagic activity in primary rat hepatocellular carcinoma and ascites hepatoma cells. Carcinogenesis1993; 14:2501-5.;Kamada Y, Funakoshi T, Shintani T, et al. Tor-mediated induction of autophagy via Apg1 protein kinase complex. J. Cell. Biol.2000; 150:1507-13.;Mizushima N, Noda T, and Ohsumi Y. Apg16p is required for the function of the apg12p-apg5p conjugate in the yeast autophagy pathway. EMBO J.1999; 18:3888-96. |
Gene Symbol | ATG16L1 |
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Gene Full Name | autophagy related 16 like 1 |
Alias Symbols | APG16L;APG16L beta;APG16-like 1;ATG16 autophagy related 16-like 1;ATG16A;ATG16L;autophagy-related protein 16-1;IBD10;WD repeat domain 30;WDR30. |
NCBI Gene Id | 55054 |
Protein Name | Autophagy-related protein 16-1|autophagy-related protein 16-1 isoform 1 [Homo sapiens] |
Description of Target | Plays an essential role in autophagy: interacts with ATG12-ATG5 to mediate the conjugation of phosphatidylethanolamine (PE) to LC3 (MAP1LC3A, MAP1LC3B or MAP1LC3C), to produce a membrane-bound activated form of LC3 named LC3-II. Thereby, controls the elongation of the nascent autophagosomal membrane (PubMed:24553140, PubMed:23376921, PubMed:24954904, PubMed:27273576, PubMed:23392225). Regulates mitochondrial antiviral signaling (MAVS)-dependent type I interferon (IFN-I) production (PubMed:25645662). Negatively regulates NOD1- and NOD2-driven inflammatory cytokine response (PubMed:24238340). Instead, promotes with NOD2 an autophagy-dependent antibacterial pathway (PubMed:20637199). Plays a role in regulating morphology and function of Paneth cell (PubMed:18849966). |
Uniprot ID | Q676U5 |
Protein Accession # | https://www.ncbi.nlm.nih.gov/protein/NP_110430 |
Nucleotide Accession # | NM_001017343.2 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "atg16 Antibody (OAPB00616)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human|Mouse|Rat".
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How long will it take to receive "atg16 Antibody (OAPB00616)"?
This item is available "Domestic: within 1-2 weeks delivery | International: 1-2 weeks".
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What buffer format is "atg16 Antibody (OAPB00616)" provided in?
This item is provided in "Liquid. ATG16 Antibody is supplied in PBS containing 0.02% sodium azide.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "atg16 Antibody (OAPB00616)"?
This target may also be called "APG16L;APG16L beta;APG16-like 1;ATG16 autophagy related 16-like 1;ATG16A;ATG16L;autophagy-related protein 16-1;IBD10;WD repeat domain 30;WDR30." in publications.
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What is the shipping cost for "atg16 Antibody (OAPB00616)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "atg16 Antibody (OAPB00616)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "atg16 Antibody (OAPB00616)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "atg16 Antibody (OAPB00616)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "ATG16L1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "ATG16L1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "ATG16L1"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "ATG16L1"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "ATG16L1"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "ATG16L1"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.