Size:50ug
$200.00
Discontinued
SKU
OASA06996
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ATG12 Antibody (OASA06996)

Catalog#: OASA06996
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This product is Genway GWB-AC296A
More Information
Predicted Species Reactivity Human, Mouse, Rat
Product Format Purified IgG - liquid
Clonality Polyclonal
Isotype Polyclonal IgG
Host Rabbit
Application IHC-FFPE, WB
Additional Information Histology Positive Control Tissue: Human brain
:: Preservative Stabilisers: 0.02% Sodium Azide (NaN3)
Antiserum Preparation: Antiserum to human ATG12 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
:: Approx Protein Conc: IgG concentration 1.0mg/ml
Buffer Solutions: Phosphate buffered saline
Reconstitution and Storage Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Replacement Item This antibody may replace item sc-271688 from Santa Cruz Biotechnology.
Immunogen The immunogen for anti-ATG12 antibody: synthetic peptide corresponding to a 15 aa sequence from near the centre of human ATG12.
Predicted Homology Based on Immunogen Sequence Human; Mouse; Rat
Datasheets/Manuals Printable datasheet for anti-ATG12 antibody - OASA06996
Specificity Rabbit anti ATG12 (Internal Region) antibody recognizes human Autophagy-related protein 12 (ATG12).
ATG12, a member of the autophagy protein family, forms a conjugate with ATG5; this conjugate has a ubiquitin-protein ligase (E3)-like activity for protein lipidation in autophagy. This conjugate also associates with innate immune response proteins such as RIG-I and VISA, inhibiting type I interferon production and permitting viral replication in host cells. ATG12 has also been shown to interact with ATG10 in human embryonic kidney cells in the presence of ATG7.
There are at least two isoforms of ATG12.
Application Info Application                       Suggested Dillution
IHC-Paraffin*                          2.5 ug/ml
Western Blot                           1  ug/ml 
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.  Sodium citrate buffer pH 6.0 is recommended for this purpose.
Western Blot detects a band of approximately 40 kD in mouse heart tissue lysate.
Publications

Bhowmik, T; Gomes, A; NKCT1 (purified Naja kaouthia protein toxin) conjugated gold nanoparticles induced Akt/mTOR inactivation mediated autophagic and caspase 3 activated apoptotic cell death in leukemic cell. 121, 86-97 (2016). IHC-FFPE, WB, Human, Mouse, Rat 27527270

Gene Symbol ATG12
Alias Symbols APG12, FBR93, APG12L, HAPG12, ATG12
NCBI Gene Id 9140
Protein Name Ubiquitin-like protein ATG12
Description of Target RABBIT ANTI ATG12 (INTERNAL REGION)
Swissprot Id O94817
Protein Accession # NP_004698.3
Protein Size (# AA) 140
Tissue Tool Find tissues and cell lines supported by DNA array analysis to express ATG12.
RNA Seq Find tissues and cell lines supported by RNA-seq analysis to express ATG12.
  1. What is the species homology for "ATG12 Antibody (OASA06996)"?

    The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human, Mouse, Rat".

  2. How long will it take to receive "ATG12 Antibody (OASA06996)"?

    This item is available "Domestic: within 1 week delivery | International: 1 week".

  3. What buffer format is "ATG12 Antibody (OASA06996)" provided in?

    This item is provided in "Purified IgG - liquid ".
    Additional format options may be available. For more information please contact info@avivasysbio.com.

  4. What are other names for "ATG12 Antibody (OASA06996)"?

    This target may also be called "APG12, FBR93, APG12L, HAPG12, ATG12" in publications.

  5. What is the shipping cost for "ATG12 Antibody (OASA06996)"?

    The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.

  6. What is the guarantee for "ATG12 Antibody (OASA06996)"?

    All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.

  7. Can I get bulk pricing for "ATG12 Antibody (OASA06996)"?

    You can get bulk pricing for this item by going here.

  8. What is the molecular weight of the protein?

    The molecular weight reported by Uniprot for this item is "".
    Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics.

  9. What protocols are available for "ATG12 Antibody (OASA06996)"?

    We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data tab on the product page.

  10. What are positive controls for "ATG12"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  11. What are negative controls for "ATG12"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  12. What other proteins interact with "ATG12"?

    This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.

  13. What biological processes are associated with "ATG12"?

    This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.

  14. What cellular components are associated with "ATG12"?

    This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.

  15. What protein functions are associated with "ATG12"?

    This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.

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