ATF5 Antibody - middle region (ARP57977_P050)

Catalog#: ARP57977_P050

• Size: 100 ul

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rat, Zebrafish
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Clonality: Polyclonal
• Host: Rabbit
• Application: WB
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Gene Symbol: ATF5
• Official Gene Full Name: Activating transcription factor 5
• NCBI Gene Id: 22809
• Protein Name: Cyclic AMP-dependent transcription factor ATF-5
• Swissprot Id: B3KND3
• Protein Accession #: NP_036200
• Nucleotide Accession #: NM_012068
• Alias Symbols: ATFX, FLJ34666, HMFN0395
• Replacement Item: This antibody may replace item sc-377168 from Santa Cruz Biotechnology.
• Description of Target: ATF5 belongs to the bZIP family. It is transcriptional activator which binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters and blocks the differentiation of neuroprogenitor cells into neurons. Its transcriptional activity is enhanced by CCND3 and slightly inhibited by CDK4. Loss of ATF5 is associated with hepatocellular carcinoma.
• Protein Size (# AA): 282
• Molecular Weight: 31kDa
• Purification: Affinity Purified
• RNA Seq: Find tissues and cell lines supported by RNA-seq analysis to express ATF5.
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human ATF5
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rat: 100%; Zebrafish: 93%
• Peptide Sequence: Synthetic peptide located within the following region: LPPPQQPPPPSPPQPSRLAPYPHPATTRGDRKQKKRDQNKSAALRYRQRK
• Concentration: Batch dependent within range: 100 ul at 0.5 - 1 mg/ml
• Protein Interactions: CEBPE; CEBPA; GIT2; ATXN1; EP300; CCND3; GABBR2; DISC1; GABBR1; CEBPG; CDC34; TRIB3; UBC; CD34;
• Blocking Peptide: For anti-ATF5 (ARP57977_P050) antibody is Catalog # AAP57977 (Previous Catalog # AAPP32388)
• Target Reference: Zhou,D., (2008) J. Biol. Chem. 283 (11), 7064-7073
• Publications:

  Wei, Y. et al. Cdc34-mediated degradation of ATF5 is blocked by cisplatin. J. Biol. Chem. 283, 18773-81 (2008). WB, Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rat, Zebrafish 18458088

Protocol:

• Reconstitution Instructions
• Immunohistochemistry (IHC) Protocol
• Immunocytochemistry (ICC) Protocol
• Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
• Western Blotting/Immunoblotting (WB/IB) Protocol
• Blocking Peptide Competition Protocol (BPCP)
• Immunoprecipitation (IP) Protocol

Tips Information:

• IHC Tips & Tricks
• ICC Tips & Tricks
• ELISA Tips & Tricks
• WB/IB Tips & Tricks
• IP Tips & Tricks

See our General FAQ page.

Product Protocols: ATF5 antibody tested with Human Fetal Liver Tissue (ARP57977_P050)