ATF5 Antibody - middle region (ARP57977_P050)

Size:100 ul

Human Liver

Basic Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rat, Zebrafish
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Clonality: Polyclonal
• Host: Rabbit
• Application: WB
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Replacement Item: This antibody may replace item sc-377168 from Santa Cruz Biotechnology.
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human ATF5
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rat: 100%; Zebrafish: 93%
• Complete computational species homology data: Anti-ATF5 (ARP57977_P050)
• Peptide Sequence: Synthetic peptide located within the following region: LPPPQQPPPPSPPQPSRLAPYPHPATTRGDRKQKKRDQNKSAALRYRQRK
• Concentration: Batch dependent within range: 100 ul at 0.5 - 1 mg/ml
• Blocking Peptide: For anti-ATF5 (ARP57977_P050) antibody is Catalog # AAP57977 (Previous Catalog # AAPP32388)
• Datasheets/Manuals: Printable datasheet for anti-ATF5 (ARP57977_P050) antibody
• Target Reference: Zhou,D., (2008) J. Biol. Chem. 283 (11), 7064-7073
• Publications:

  Wei, Y. et al. Cdc34-mediated degradation of ATF5 is blocked by cisplatin. J. Biol. Chem. 283, 18773-81 (2008). WB, Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rat, Zebrafish 18458088

Target Info

• Gene Symbol: ATF5
• Official Gene Full Name: Activating transcription factor 5
• Alias Symbols: ATFX, FLJ34666, HMFN0395
• NCBI Gene Id: 22809
• Protein Name: Cyclic AMP-dependent transcription factor ATF-5
• Description of Target: ATF5 belongs to the bZIP family. It is transcriptional activator which binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters and blocks the differentiation of neuroprogenitor cells into neurons. Its transcriptional activity is enhanced by CCND3 and slightly inhibited by CDK4. Loss of ATF5 is associated with hepatocellular carcinoma.
• Swissprot Id: B3KND3
• Protein Accession #: NP_036200
• Nucleotide Accession #: NM_012068
• Protein Size (# AA): 282
• Molecular Weight: 31kDa
• Tissue Tool: Find tissues and cell lines supported by DNA array analysis to express ATF5.
• RNA Seq: Find tissues and cell lines supported by RNA-seq analysis to express ATF5.
• Protein Interactions: CEBPE; CEBPA; GIT2; ATXN1; EP300; CCND3; GABBR2; DISC1; GABBR1; CEBPG; CDC34; TRIB3; UBC; CD34;