ATF5 Antibody (Acetyl-Lys29) (OAAF08212)

Catalog No: OAAF08212

Size:100 ug

Western blot analysis of lysate from A549 cells, using ATF5 (Acetyl-Lys29) Antibody.

Product Info

• Tested Species Reactivity: Human, Mouse, Rat
• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid. PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Western blot
• Additional Information: Modification Sites: Human:K29, Mouse:K29, Rat:K29
• Reconstitution and Storage: -20°C
• Immunogen: The antiserum was produced against synthesized Acetyl-peptide derived from human ATF5 around the Acetylation site of Lys29.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using acetylated peptide. The antibody against non-acetylated peptide was removed by chromatography using corresponding non-acetylated peptide.
• Concentration: 1 mg/ml
• Target Post-Translational Modification: Acetyl
• Specificity: ATF5 (Acetyl-Lys29) Antibody detects endogenous levels of ATF5 protein only when acetylated at Lys29.
• Application Info: WB: 1:500~1:1000
  ELISA: 1:10000
  Optimal dilutions should be determined by the end user.

Target Info

• Gene Symbol: ATF5
• Gene Full Name: activating transcription factor 5
• Alias Symbols: Activating transcription factor 5;ATFX;cAMP-dependent transcription factor ATF-5;cyclic AMP-dependent transcription factor ATF-5;HMFN0395;transcription factor ATFx.
• NCBI Gene Id: 22809
• Protein Name: Cyclic AMP-dependent transcription factor ATF-5
• Description of Target: Transcription factor that either stimulates or represses gene transcription through binding of different DNA regulatory elements such as cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), ATF5-specific response element (ARE) (consensus: 5'-C[CT]TCT[CT]CCTT[AT]-3') but also the amino acid response element (AARE), present in many viral and cellular promoters. Critically involved, often in a cell type-dependent manner, in cell survival, proliferation, and differentiation (PubMed:10373550, PubMed:15358120, PubMed:21212266, PubMed:20654631). Its transcriptional activity is enhanced by CCND3 and slightly inhibited by CDK4 (PubMed:15358120). Important regulator of the cerebral cortex formation, functions in cerebral cortical neuroprogenitor cells to maintain proliferation and to block differentiation into neurons. Must be down-regulated in order for such cells to exit the cycle and differentiate (By similarity). Participates in the pathways by which SHH promotes cerebellar granule neuron progenitor cells proliferation (By similarity). Critical for survival of mature olfactory sensory neurons (OSN), directs expression of OSN-specific genes (By similarity). May be involved in osteogenic differentiation (PubMed:22442021). Promotes cell proliferation and survival by inducing the expression of EGR1 sinergistically with ELK1. Once acetylated by EP300, binds to ARE sequences on target genes promoters, such as BCL2 and EGR1 (PubMed:21791614). Plays an anti-apoptotic role through the transcriptional regulation of BCL2, this function seems to be cell type-dependent (By similarity). Cooperates with NR1I3/CAR in the transcriptional activation of CYP2B6 in liver (PubMed:18332083). In hepatic cells, represses CRE-dependent transcription and inhibits proliferation by blocking at G2/M phase (PubMed:22528486, PubMed:18701499). May act as a negative regulator of IL1B transduction pathway in liver (PubMed:24379400). Upon IL1B stimulus, cooperates with NLK to activate the transactivation activity of C/EBP subfamily members (PubMed:25512613). Besides its function of transcription factor, acts as a cofactor of CEBPB to activate CEBPA and promote adipocyte differentiation (PubMed:24216764). Regulates centrosome dynamics in a cell-cycle- and centriole-age-dependent manner. Forms 9-foci symmetrical ring scaffold around the mother centriole to control centrosome function and the interaction between centrioles and pericentriolar material (PubMed:26213385).
• Uniprot ID: Q9Y2D1
• Molecular Weight: 30 kDa