ATF4 Antibody (Phospho-Ser219) (OASG01829)

Catalog No: OASG01829

Size:100 uL

Western Blot using COLO205

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid. PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: WB, ELISA
• Additional Information: Cellular Localization: Cytoplasm Cell membrane Nucleus Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Colocalizes with GABBR1 in hippocampal neuron dendritic membranes . Colocalizes with NEK6 at the centrosome.
• Reconstitution and Storage: Store at -20°C. Avoid repeated freeze/thaw cycles.
• Immunogen: Synthesized peptide derived between 160-240aa from human CREB-2 around the phosphorylation site of S219.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Concentration: 1 mg/ml
• Target Post-Translational Modification: Ubiquitinated by SCF(BTRC) in response to mTORC1 signal, followed by proteasomal degradation and leading to down-regulate expression of SIRT4. Phosphorylated by NEK6 . Phosphorylated on the betaTrCP degron motif at Ser-219, followed by phosphorylation at Thr-213, Ser-224, Ser-231, Ser-235 and Ser-248, promoting interaction with BTRC and ubiquitination. Phosphorylation is promoted by mTORC1 . Phosphorylated by NEK6.
• Specificity: Phospho-CREB-2 (S219) Polyclonal Antibody detects endogenous levels of CREB-2 protein only when phosphorylated at S219.
• Application Info: WB: 1:500-1:2000
  ELISA: 1:10000

Target Info

• Gene Symbol: ATF4
• Gene Full Name: activating transcription factor 4
• Alias Symbols: CREB2, TXREB, CREB-2, TAXREB67
• NCBI Gene Id: 468; 11911;
• Description of Target: This gene encodes a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain. Two alternative transcripts encoding the same protein have been described. Two pseudogenes are located on the X chromosome at q28 in a region containing a large inverted duplication.
• Uniprot ID: P18848, Q06507
• Molecular Weight: 38 kDa