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- Phone: 858-552-6979
- Email: info@avivasysbio.com
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
| Datasheets/Manuals | Printable datasheet for OASE00295-FITC |
|---|
| Predicted Species Reactivity | Human, Rat |
|---|---|
| Product Format | Liquid. Purified antibody supplied in 1x PBS buffer. |
| Clonality | Monoclonal |
| Clone | S360A-24 |
| Isotype | IgG2a |
| Host | Mouse |
| Conjugation | FITC: Fluorescein Isothiocyanate |
| Application | WB, ICC, IHC |
| Additional Information | Background Info: Detects 60 kDa protein. Does not cross-react with ATF5 |
| :: | Scientific Background: Cyclic AMP-dependent transcription factor ATF-4 (ATF4) is a basic leucine-zipper (bZip) transcription factor, which regulates amino acid metabolism, DNA damage repair, chromatin remodeling, and apoptosis in response to cellular and ER stress. ATF4 works with various proteins, such as C/EBP homology protein (CHOP), aspargine synthetase (ASNS), and cAMP response element (CRE) among others to mediate cellular stress. ATF4 also regulates glucose homeostasis by suppressing beta-cell proliferation and insulin production. Furthermore, ATF4 targets the histone demethylase JMJD3 to alter chromatin structure and enhance gene transcription in response to amino acid deprivation. |
| :: | Certificate of Analysis: 1ug/mL was sufficient for detection of ATF4 in 20 ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. |
| Reconstitution and Storage | All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil). Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding. |
| Immunogen | Fusion protein amino acids 25-327 (nearly full-length, all but first 24 amino acids) of human ATF4 (accession # P18848). 86% identical to rat, and 85% identical to mouse. <50% identity with ATF5. |
| Purification | Protein G Purified |
| Concentration | 1 mg/ml |
| Dilution | 1:1000 WB |
| Storage Buffer | PBS pH 7.4, 50% glycerol, 0.1% sodium azide |
| Gene Symbol | ATF4 |
|---|---|
| Gene Full Name | activating transcription factor 4 |
| Alias Symbols | CREB2, TXREB, CREB-2, TAXREB67 |
| NCBI Gene Id | 468 |
| Uniprot ID | P18848 |
| Protein Accession # | NP_001666.2 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "ATF4 Antibody : FITC (OASE00295-FITC)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human, Rat".
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How long will it take to receive "ATF4 Antibody : FITC (OASE00295-FITC)"?
This item is available "Domestic: within 3-5 days delivery | International: 3-5 days".
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What buffer format is "ATF4 Antibody : FITC (OASE00295-FITC)" provided in?
This item is provided in "Liquid. Purified antibody supplied in 1x PBS buffer.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "ATF4 Antibody : FITC (OASE00295-FITC)"?
This target may also be called "CREB2, TXREB, CREB-2, TAXREB67" in publications.
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What is the shipping cost for "ATF4 Antibody : FITC (OASE00295-FITC)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "ATF4 Antibody : FITC (OASE00295-FITC)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "ATF4 Antibody : FITC (OASE00295-FITC)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "ATF4 Antibody : FITC (OASE00295-FITC)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "ATF4"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "ATF4"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "ATF4"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "ATF4"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "ATF4"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "ATF4"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.
