ATF3 ELISA Kit (Mouse) (OKDD00746)

Catalog No: OKDD00746 (Formerly GWB-KBBIT5)

Size:96 Wells

Mouse ATF3 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked immunosorbent assay
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: ~4 Hours
• ELISA Kit Principle: Aviva Systems Biology ATF3 ELISA Kit (Mouse) (OKDD00746) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for ATF3 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ATF3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample ATF3 captured in well.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility:

  Intra-assay Precision: 3 samples with low, middle and high level analyte were measured with 20 replicates on one plate, respectively.

  Inter-assay Precision: 3 samples with low, middle and high level analyte were measured across 3 different plates, with 8 replicates per plate.

  Mean Intra-Assay: CV<10%

  Mean Inter-Assay: CV<12%

• ELISA Kit Component:
  • Anti-ATF3 Microplate: 96 Wells (12 x 8 Well Strips)
  • ATF3 Lyophilized Standard: 2 x 10 ng
  • Standard Diluent: 1 x 20 mL
  • 100X ATF3 Biotinylated Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Detection Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • Detection Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue homogenates, cell lysates
• Sensitivity: <0.063ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of ATF3. No significant cross-reactivity or interference between ATF3 and analogues was observed.
• Assay Info: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Atf3
• Gene Full Name: activating transcription factor 3
• Alias Symbols: Activating transcription factor 3;cAMP-dependent transcription factor ATF-3;cyclic AMP-dependent transcription factor ATF-3;LRG-;LRG-21;transcription factor LRG-21.
• NCBI Gene Id: 11910
• Protein Name: Cyclic AMP-dependent transcription factor ATF-3
• Description of Target: This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Represses transcription from promoters with ATF sites. It may repress transcription by stabilizing the binding of inhibitory cofactors at the promoter (By similarity).
• Uniprot ID: Q60765
• Protein Accession #: NP_031524.2
• Nucleotide Accession #: NM_007498.3
• Protein Size (# AA): 181
• Molecular Weight: 21 kDa