Catalog No: OKAG00366
Size:12x8WELLSTRIPS
Price: $600.00
SKU
OKAG00366
Availability: Domestic: within 1-2 week delivery | International: 1-2 weeks
Contact Us:
- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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Predicted Species Reactivity | Human, Mouse, Rat | ||||||||||||||||||||||||||||||||||
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Product Format | 12 x 8-Well Microstrips | ||||||||||||||||||||||||||||||||||
Application | Enzyme-linked immunosorbent assay | ||||||||||||||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric 450 nm | ||||||||||||||||||||||||||||||||||
ELISA Kit Principle | The Aviva DNA-Binding ELISA Kit contains components necessary for detection of active transcription factors in eukaryotic nuclear or cell lysates. This particular immunoassay utilizes the qualitative technique of an indirect ELISA. Streptavidin is bound to the immunoassay plate and specific biotinylated double-stranded (dsDNA) oligonucleotides are then added to bind to the streptavidin via a high affinity biotin-streptavidin interaction. After subsequent blocking of extraneous binding sites in each well, the sample containing the target of interest can be added. Primary antibody is added to bind activated transcription factorsbound to the dsDNA oligonucleotide, which has been immobilized via the plate-coated streptavidin. A HRP-conjugated secondary antibody specific for rabbit IgGs is added, which allows for specific binding to the Primary Antibody, and consequently colorimetric detection upon addition of the TMB substrate. For color development, TMB(3, 3’, 5, 5’-Tetramethylbenzidine) is added to each well. After addition of the substrate, a peroxidase catalyzed reaction will produce a blue TMB Diimine product that is proportional to the target concentration in the sample. Color development is quenched by addition of Stop Solution, or2N Sulfuric Acid, which turns the solution yellow. The absorbance can then be read by a spectrophotometer at 450nm and subsequentlyallowing for determination of the target concentration in the sample. | ||||||||||||||||||||||||||||||||||
ELISA Kit Component |
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:: | OMIM: 603148 UniGene: Hs.460 | ||||||||||||||||||||||||||||||||||
:: | Note: The product is for research use only, not for use in diagnostic or therapeutic procedures. | ||||||||||||||||||||||||||||||||||
Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||||||||||||||
Specificity | The ATF3 DNA-Binding ELISA Kit detects endogenous levels of total ATF3 protein. | ||||||||||||||||||||||||||||||||||
Assay Info | Assay Type: Qualitative DNA Binding ELISA |
Gene Symbol | ATF3 |
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Gene Full Name | activating transcription factor 3 |
Alias Symbols | Activating transcription factor 3, cAMP-dependent transcription factor ATF-3, Cyclic AMP-dependent transcription factor ATF-3 |
NCBI Gene Id | 467 |
Protein Name | cyclic AMP-dependent transcription factor ATF-3 |
Description of Target | This gene encodes a member of the mammalian activation transcription factor/cAMP responsive element-binding (CREB) protein family of transcription factors. This gene is induced by a variety of signals, including many of those encountered by cancer cells, and is involved in the complex process of cellular stress response. Multiple transcript variants encoding different isoforms have been found for this gene. It is possible that alternative splicing of this gene may be physiologically important in the regulation of target genes. |
Uniprot ID | P18847 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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