ATF2 ELISA Kit (Chicken) (OKEH08705)

Catalog No: OKEH08705

Size:96T

Product Info

• Predicted Species Reactivity: Chicken, Gallus gallus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology ATF2 ELISA Kit (Chicken) (OKEH08705) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ATF2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ATF2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ATF2 captured in well.
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 80% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=7.6% (n = 20)
  Mean Inter-assay CV%: <=10.3% (n = 20)
• ELISA Kit Component:
  • ATF2 Microplate: 96 Wells (12 x 8 Well strips)
  • ATF2 Lyophilized Standard: 2
  • 100X Biotinylated ATF2 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Transcriptional activator which regulates the transcription of various genes, including those involved in anti-apoptosis, cell growth, and DNA damage response. Dependent on its binding partner, binds to CRE (cAMP response element) consensus sequences (5'-TGACGTCA-3') or to AP-1 (activator protein 1) consensus sequences (5'-TGACTCA-3').
  Subcellular Location: Nucleus Cytoplasm Mitochondrion outer membrane
  Unigene: Gga.27381
  String: 9031.ENSGALP00000032623
  KEGG: gga:395727
  Pfam: PF00170
• Reconstitution and Storage: Store as indicated in product manual.
• Specificity: Natural and recombinant Chicken Cyclic AMP-dependent transcription factor ATF-2
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: ATF2
• Gene Full Name: activating transcription factor 2
• Alias Symbols: Activating transcription factor 2;cAMP-dependent transcription factor ATF-2;cyclic AMP-dependent transcription factor ATF-2.
• NCBI Gene Id: 395727
• Protein Name: O93602
• Description of Target: Cyclic AMP-dependent transcription factor ATF-2
• Uniprot ID: https://www.uniprot.org/uniprot/O93602
• Protein Accession #: NP_990235.1
• Nucleotide Accession #: NM_204904.1