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ATF2 Antibody (OAEB01901)

100 ug
$349.00
In Stock
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Gene Symbol:
ATF2
NCBI Gene Id:
1386
Protein Name:
Cyclic AMP-dependent transcription factor ATF-2
Swissprot Id:
P15336
Protein Accession #:
NP_001871.2
Alias Symbols:
ATF2, activating transcription factor 2, HB16, CREB2, TREB7, CRE-BP1, cAMP responsive element binding protein 2, MGC111558, activating transcription factor 2 splice variant ATF2-var2, cAMP responsive element binding protein 2, formerly
Replacement Item:
This antibody may replace item sc-126459 from Santa Cruz Biotechnology.
Host:
Goat
Clonality:
Polyclonal
Application:
WB
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express ATF2.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express ATF2.
Predicted Species Reactivity:
Cow, Horse, Human, Mouse, Pig, Rabbit, Rat
Predicted Homology Based on Immunogen Sequence:
Cow: 100%; Horse: 85%; Human: 100%; Mouse: 85%; Pig: 100%; Rabbit: 100%; Rat: 92%
Complete computational species homology data:
ATF2 Antibody (OAEB01901)
Peptide Sequence:
C-IVMAPSSQSQPSGS
Product Format:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Reconstitution and Storage:
Aliquot and store at -20C. Minimize freezing and thawing.
Datasheets/Manuals:
Printable datasheet for anti-ATF2 antibody - OAEB01901
Purity:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Application Info:
Peptide ELISA: antibody detection limit dilution 1:32000.
Western Blot: Experiments gave bands at approx 55kDa and 30kDa in lysates of Human Placenta and of cell line HeLa after 0.5ug/ml antibody staining. These bands correspond to earlier findings in literature with different antibodies (Bailey et al, J Clin Endocrinol Metab. 2002 Apr;87(4):1717-28.; PMID: 11932306). This protein has a calculated MW of 54.5kDa according to NP_001871.2. Recommended concentration: 0.5-1.5ug/ml.
Target Reference:
* Monzen K, Hiroi Y, Kudoh S, Akazawa H, Oka T, Takimoto E, Hayashi D, Hosoda T, Kawabata M, Miyazono K, Ishii S, Yazaki Y, Nagai R, Komuro I.; Smads, TAK1, and their common target ATF-2 play a critical role in cardiomyocyte differentiation.; J Cell Biol 2001 May 14;153(4):687-98; Pubmed 11352931

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