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ATF1 ELISA Kit (Human) (OKCD07343)

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Application:
ELISA
Gene Symbol:
ATF1
Official Gene Full Name:
activating transcription factor 1
NCBI Gene Id:
466
Alias Symbols:
EWS-ATF1; FUS/ATF-1; TREB36; Cyclic AMP-Dependent Transcription Factor
Description of Target:
ATF1 binds the cAMP response element (CRE) (consensus: 5'-GTGACGT..-3'), a sequence present in many viral and cellular promoters. ATF1 binds to the Tax-responsive element (TRE) of HTLV-I. ATF1 mediates PKA-induced stimulation of CRE-reporter genes.
Protein Name:
Cyclic AMP-dependent transcription factor ATF-1
Swissprot Id:
P18846
Protein Accession #:
NM_005171.4
Nucleotide Accession #:
NP_005162.1
Predicted Species Reactivity:
Human
Sample Type:
Tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity:
< 0.066ng/mL
Kit Range:
0.156-10ng/mL
Kit Reproducibility:
Intra-assay reproducibility was evaluated using 20 replicates of 3 samples representing low, middle and high level ATF1. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level ATF1 using 8 replicates on each of 3 plates.
Intra-Assay CV <10%
Inter-Assay CV <12%
Kit Duration:
~ 3 Hours
Kit Principle:
Aviva Systems Biology ATF1 ELISA Kit (Human) (OKCD07343) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ATF1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ATF1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ATF1 captured in the well.
Kit Component:
ComponentAmount
ATF1 Microplate96 Wells (12 x 8 Well strips)
ATF1 Lyophilized Standard2
100X ATF1 HRP-Detector Anitbody1 x 120 µL
100X Avidin-HRP Conjugate1 x 120 µL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by RNA-seq analysis to express ATF1.
RNA Seq:
Find tissues and cell lines supported by DNA array analysis to express ATF1.
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Reconstitution and Storage:
Store as indicated in product manual.

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