ASS1 ELISA Kit (Chicken) (OKEH08792)

Catalog No: OKEH08792

Size:96T

Product Info

• Predicted Species Reactivity: Chicken, Gallus gallus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology ASS1 ELISA Kit (Chicken) (OKEH08792) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ASS1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ASS1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ASS1 captured in well.
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 111% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=4.9% (n = 20)
  Mean Inter-assay CV%: <=10.3% (n = 20)
• ELISA Kit Component:
  • ASS1 Microplate: 96 Wells (12 x 8 Well strips)
  • ASS1 Lyophilized Standard: 2
  • 100X Biotinylated ASS1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
  
  Unigene: Gga.5385
  SMR: Q5ZJ23
  String: 9031.ENSGALP00000006379
  KEGG: gga:417185
  Pfam: PF00764
• Reconstitution and Storage: Store as indicated in product manual.
• Specificity: Natural and recombinant Chicken Argininosuccinate synthase
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: ASS1
• Gene Full Name: argininosuccinate synthase 1
• Alias Symbols: argininosuccinate synthase;argininosuccinate synthetase 1;citrulline--aspartate ligase.
• NCBI Gene Id: 417185
• Protein Name: Q5ZJ23
• Description of Target: Argininosuccinate synthase
• Uniprot ID: https://www.uniprot.org/uniprot/Q5ZJ23
• Protein Accession #: NP_001013413.1
• Nucleotide Accession #: NM_001013395.1