ASS1 Antibody - C-terminal region (OAAB08885)

Catalog No: OAAB08885

Size:400ul

Western blot analysis of ASS1 (arrow) using rabbit polyclonal ASS1 Antibody (C-term) (Cat. #OAAB08885). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ASS1 gene.

ASS1 is strongly supported by BioGPS gene expression data to be expressed in HEK293, Jurkat

ASS1 Antibody (C-term) (Cat. #OAAB08885) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the ASS1 antibody detected the ASS1 protein (arrow).

ASS1 Antibody (C-term) (Cat. #OAAB08885)immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ASS1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

ASS1 Antibody (C-term) (Cat. #OAAB08885) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid. PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB, IHC-P, FC
• Additional Information: Function: Is indirectly involved in the control of blood pressure (By similarity).
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This ASS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 281-310 amino acids from the C-terminal region of human ASS1.
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Predicted Homology Based on Immunogen Sequence: Bovine, Chicken, Mouse, Rat, Rabbit
• Intended Use: ASS1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
• Application Info: WB: 1:1000
  IHC-P: 1:10~50
  FC: 1:10~50
• Reference: Hozyasz, K.K., et al. Arch. Oral Biol. 55(11):861-866(2010)
  Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
  Kobayashi, E., et al. Mol. Cancer Ther. 9(3):535-544(2010)
  Tsai, W.B., et al. Mol. Cancer Ther. 8(12):3223-3233(2009)
  Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)

Target Info

• Gene Symbol: ASS1
• Alias Symbols: ASS, CTLN1
• NCBI Gene Id: 445
• Protein Name: Argininosuccinate synthase
• Description of Target: The protein encoded by this gene catalyzes the penultimate step of the arginine biosynthetic pathway. There are approximately 10 to 14 copies of this gene including the pseudogenes scattered across the human genome, among which the one located on chromosome 9 appears to be the only functional gene for argininosuccinate synthetase. Mutations in the chromosome 9 copy of ASS cause citrullinemia. Two transcript variants encoding the same protein have been found for this gene.
• Uniprot ID: P00966
• Protein Accession #: NP_000041.2
• Molecular Weight: 46530 Da