Catalog No: OKEH02654
Size:96T
Price: $625.00
SKU
OKEH02654
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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ASRGL1 ELISA Kit (Bovine) : 96 Wells (OKEH02654)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityBos taurus, Bovine
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology ASRGL1 ELISA Kit (Bovine) (OKEH02654) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ASRGL1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for ASRGL1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ASRGL1 captured in well.
ELISA Kit Range78-5000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 93%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.3% (n = 20)
Mean Inter-assay CV%: < 7.8% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-ASRGL1 Microplate96 Wells (12 x 8 Well strips)
ASRGL1 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated ASRGL1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity26 pg/mL
SpecificityNatural and recombinant Bovine Isoaspartyl peptidase/L-asparaginase
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolASRGL1
Gene Full Nameasparaginase and isoaspartyl peptidase 1
Alias Symbolsasparaginase like 1;asparaginase-like protein 1;beta-aspartyl-peptidase;isoaspartyl dipeptidase;isoaspartyl peptidase/L-asparaginase;L-asparaginase;L-asparagine amidohydrolase.
NCBI Gene Id767970
Protein NameQ32LE5
Description of TargetIsoaspartyl peptidase/L-asparaginase
Uniprot IDhttps://www.uniprot.org/uniprot/Q32LE5
Protein Accession #NP_001070503.1
Nucleotide Accession #NM_001077035.2
Protein Size (# AA)308
Molecular Weight32 kDa
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