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Datasheets/Manuals | Printable datasheet for ASPERGILLUS SPP Antibody (OASA00216) |
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Predicted Species Reactivity | Aspergillus |
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Product Format | Liquid. Phosphate buffered saline |
Clonality | Monoclonal |
Clone | WF-AF-1 |
Isotype | IgM |
Host | Mouse |
Application | Immunohistochemistry-Paraffin|Immunoprecipitation|Western blot |
Additional Information | Fusion Partners: Spleen cells from immunized Balb/c ABom mice were fused with cells of the X63-Ag8.653 myeloma cell line. Histology Positive Control Tissue: Aspergillus infected placenta. |
Reconstitution and Storage | -20°C |
Immunogen | Wall fraction (WF) of Aspergillus fumigatus |
Purification | Purified IgM prepared by ammonium sulfate precipitation from tissue culture supernatant |
Predicted Homology Based on Immunogen Sequence | Fungal |
Concentration | 1 mg/ml |
Specificity | Mouse anti Aspergillus spp., clone WF-AF-1, is raised against the wall fraction (WF) of Aspergillus fumigatus. This antibody specifically recognizes members of the Aspergillus spp. including A. flavus and A. niger, reacting strongly with walls and septae, and to a lesser extent within the cytoplasm of the hyphae. Mouse anti Aspergillus spp., clone WF-AF-1 does not bind to water-soluble somatic antigens (WSSA) of Aspergillus spp., but may react with galactomannans of members of the genus Penicillium. |
Application Info | IHC-P: 1/300. This product requires protein digestion pre-treatment of paraffin sections WB: Mouse anti Aspergillus spp antibody, clone WF-AF-1 detects a band of approximately 106 kDa of Aspergillus fumigatus wall fraction (WF). |
Reference | 1. Jensen, H.E. et al. (1996) Development of murine monoclonal antibodies for the immunohistochemical diagnosis of systemic bovine aspergillosis. J. Vet. Diagn. Invest. 8: 68-75. 2. Jensen, H.E. et al. (1996) Diagnosis of systemic mycoses by specific immunohistochemical tests. APMIS. 104: 241-258. 3. Jensen, H.E. et al. (1997) The use of immunohistochemistry to improve sensitivity and specificity in the diagnosis of systemic mycoses in patients with haematological malignancies. J. Pathol. 181: 100-105. |
Description | A. fumigatus, a thermophilic, opportunistic and angio-invasive filamentous fungus, is the main causative agent of systemic bovine aspergillosis, a worldwide and often fatal respiratory disease of cattle. Clone WF-AF-1 has been successfully used in immunohistochemistry for the specific and consistent in situ diagnosis of bovine systemic aspergillosis, attributed to its binding to the major cell wall component, galactomannan. Clone WF-AF-1 has also been used for the identification of aspergillosis in human tissue sections. |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "ASPERGILLUS SPP Antibody (OASA00216)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Aspergillus".
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How long will it take to receive "ASPERGILLUS SPP Antibody (OASA00216)"?
This item is available "Domestic: within 1-2 weeks delivery | International: 1-2 weeks".
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What buffer format is "ASPERGILLUS SPP Antibody (OASA00216)" provided in?
This item is provided in "Liquid. Phosphate buffered saline".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "ASPERGILLUS SPP Antibody (OASA00216)"?
This target may also be called "" in publications.
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What is the shipping cost for "ASPERGILLUS SPP Antibody (OASA00216)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "ASPERGILLUS SPP Antibody (OASA00216)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "ASPERGILLUS SPP Antibody (OASA00216)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "ASPERGILLUS SPP Antibody (OASA00216)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for ""?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for ""?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with ""?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with ""?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with ""?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with ""?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.