ASPA Antibody - N-terminal region (OAAB03967)

Catalog No: OAAB03967 (Formerly GWB-C5D32Q)

Size:400ul

Western blot analysis of ASPA Antibody (N-term) (Cat. #OAAB03967) in MCF7 cell line lysates (35ug/lane). ASPA (arrow) was detected using the purified Pab.

Western blot analysis of ASPA (arrow) using rabbit polyclonal ASPA Antibody (N-term) (Cat. #OAAB03967). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ASPA gene.

Formalin-fixed and paraffin-embedded human skeletal muscle reacted with ASPA Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Confocal immunofluorescent analysis of ASPA Antibody (N-term)(Cat. #OAAB03967) with MCF-7 cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Flow cytometric analysis of MDA-MB468 cells using ASPA Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB, IHC-P, FC, IF
• Additional Information: Function: Catalyzes the deacetylation of N-acetylaspartic acid (NAA) to produce acetate and L-aspartate. NAA occurs in high concentration in brain and its hydrolysis NAA plays a significant part in the maintenance of intact white matter. In other tissues it act as a scavenger of NAA from body fluids.
  Cellular Location: Cytoplasm. Nucleus.
  Tissue Location: Brain white matter, skeletal muscle, kidney, adrenal glands, lung and liver
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This ASPA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 82-110 amino acids from the N-terminal region of human ASPA.
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Predicted Homology Based on Immunogen Sequence: Human, Cow
• Intended Use: ASPA Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
• Application Info: WB: 1:1000
  IHC-P: 1:50~100
  FC: 1:10~50
  IF: 1:10~50
• Other Applications Image 1 Data: Flow cytometric analysis of MDA-MB468 cells using ASPA Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Target Info

• Gene Symbol: ASPA
• Alias Symbols: ASP, ACY2
• NCBI Gene Id: 443
• Protein Name: Aspartoacylase
• Description of Target: ASPA is an enzyme that catalyzes the conversion of N-acetyl_L-aspartic acid (NAA) to aspartate and acetate. NAA is abundant in the brain where hydrolysis by aspartoacylase is thought to help maintain white matter. This protein is an NAA scavenger in other tissues.
• Uniprot ID: P45381
• Protein Accession #: NP_000040.1
• Molecular Weight: 35735 Da