Catalog No: OKCD08493
Size:96WELLS
Price: $800.00
SKU
OKCD08493
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Datasheets/ManualsPrintable datasheet for ASMT ELISA kit (Human)
Product Info
Predicted Species ReactivityHuman
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColormetric|Regular detection
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Acetylserotonin-O-Methyltransferase (ASMT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Acetylserotonin-O-Methyltransferase (ASMT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Acetylserotonin-O-Methyltransferase (ASMT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Acetylserotonin-O-Methyltransferase (ASMT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Acetylserotonin-O-Methyltransferase (ASMT) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Acetylserotonin-O-Methyltransferase (ASMT) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
ASMT Microplate96 Wells (12 x 8 Well strips)
ASMT Lyophilized Standard2
100X ASMT HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C, -20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity< 0.054ng/mL
Gene SymbolASMT
Gene Full Nameacetylserotonin O-methyltransferase
Alias SymbolsASMTY, HIOMT, HIOMTY
NCBI Gene Id438
Protein NameAcetylserotonin O-methyltransferase
Description of TargetThis gene belongs to the methyltransferase superfamily, and is located in the pseudoautosomal region (PAR) at the end of the short arms of the X and Y chromosomes. The encoded enzyme catalyzes the final reaction in the synthesis of melatonin, and is abundant in the pineal gland. Alternatively spliced transcript variants have been noted for this gene.
Uniprot IDP46597
Protein Accession #NP_001164509.1
Nucleotide Accession #NM_001171038.1
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