ASL ELISA Kit (Human) (OKEH08308)

Catalog No: OKEH08308

Size:96T

Human ASL ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology ASL ELISA Kit (Human) (OKEH08308) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ASL has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ASL is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ASL captured in well.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 94% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=7.1% (n = 20)
  Mean Inter-assay CV%: <=11.2% (n = 20)
• ELISA Kit Component:
  • ASL Microplate: 96 Wells (12 x 8 Well strips)
  • ASL Lyophilized Standard: 2
  • 100X Biotinylated ASL Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
  
  Unigene: Hs.632015
  SMR: P04424
  String: 9606.ENSP00000307188
  KEGG: hsa:435
  MIM: 207900
  Pfam: PF14698
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 39pg/mL
• Specificity: Natural and recombinant Human Argininosuccinate lyase
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: ASL
• Gene Full Name: argininosuccinate lyase
• Alias Symbols: argininosuccinase;argininosuccinate lyase;arginosuccinase;ASAL.
• NCBI Gene Id: 435
• Protein Name: P04424
• Description of Target: Argininosuccinate lyase
• Uniprot ID: https://www.uniprot.org/uniprot/P04424
• Protein Accession #: NP_000039.2
• Nucleotide Accession #: NM_000048.3