Catalog No: OCOA00396
Size:20UG
Price: $179.00
SKU
OCOA00396
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ASIC2 Protein Lysate (OCOA00396)

Datasheets/ManualsPrintable datasheet for ASIC2 Protein Lysate (OCOA00396)
Product Info
Predicted Species ReactivityHomo sapiens
Product FormatLyophilized
HostHEK293T
Additional InformationComponents: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
::Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution and StorageThe lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
TagC-MYC/DDK
DescriptionASIC2 HEK293T cell transient overexpression lysate (as WB positive control)
Gene SymbolASIC2
Gene Full Nameacid sensing ion channel subunit 2
Alias SymbolsACCN;ACCN1;acid sensing (proton gated) ion channel 2;acid-sensing ion channel 2;Amiloride-sensitive brain sodium channel;amiloride-sensitive cation channel 1, neuronal;Amiloride-sensitive cation channel neuronal 1;ASIC2a;BNaC1;BNC1;brain sodium channel 1;hBNaC1;mammalian degenerin homolog;MDEG;neuronal amiloride-sensitive cation channel 1.
NCBI Gene Id40
Protein NameNM_001094, NP_001085, Q16515
Description of TargetAcid-sensing ion channel 2, acid-sensing ion channel 2 isoform MDEG1 [Homo sapiens], Homo sapiens acid sensing ion channel subunit 2 (ASIC2), transcript variant MDEG1, mRNA
Uniprot IDhttps://www.uniprot.org/uniprot/Q16515
Protein Accession #https://www.ncbi.nlm.nih.gov/protein/NP_001085
Nucleotide Accession #https://www.ncbi.nlm.nih.gov/nuccore/NM_001094
Molecular Weight57.5 kDa
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