ASAH1 Antibody (OAPB00726)

Catalog No: OAPB00726

Size:0.1 mg

Western blot analysis of ASAH1 in mouse heart tissue lysate with ASAH1 antibody at (A) 1 and (B) 2 ug/mL.

Immunohistochemistry of ASAH1 in human heart tissue with ASAH1 antibody at 2.5 ug/mL.

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Liquid PBS containing 0.02% sodium azide
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: WB, IHC-P, IF, ELISA
• Additional Information: Positive Control: Human heart tissue
• Reconstitution and Storage: Can be stored at 4°C for three months and -20°C stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Immunogen: Antibody was raised against a 16 amino acid peptide near the carboxy terminus of the human ASAH1. The immunogen is located within amino acids 240 - 290 of ASAH1.
• Purification: Affinity chromatography purified via peptide column
• Predicted Homology Based on Immunogen Sequence: Bovine (88%)
• Concentration: 1 mg/ml
• Specificity: Multiple isoforms of ASAH1 are known to exist.
• Application Info: WB: 1-2 ug/mL
  IHC-P: 2.5 ug/mL
  IF: 20 ug/mL
  Antibody validated: Western Blot in human samples; Immunohistochemistry in human and rat samples and Immunofluorescence in human and rat samples. All other applications and species not yet tested.
  Optimal dilutions should be determined by the end user.
• Enhanced Validation: Enhanced Validation
• Publications:

  Increased placental mitochondrial fusion in gestational diabetes mellitus: an adaptive mechanism to optimize feto-placental metabolic homeostasis?. BMJ Open Diabetes Res Care. 8, (2020). 32144130

Target Info

• Gene Symbol: ASAH1
• Alias Symbols: AC, PHP, ASAH, PHP32, ACDase, SMAPME
• NCBI Gene Id: 427
• Protein Name: Acid ceramidase
• Description of Target: Sphingolipids are hydrolyzed by ceramidases to yield sphingosine and fatty acids. These ceramidases are classified according to the pH range that supports their optimal activity. ASAH1 is an acid ceramidase and key regulator of ceramide metabolism. Mutations in this gene results in Farber Lipogranulomatosis, a fatal human genetic disorder that results in the painful swelling of the joints and tendons and pulminary insufficiency, while a complete knockout of its expression is lethal in mice. Recent studies have shown elevated levels of ASAH1 in AlzheimerÂ’s disease (AD) patients correlating with a reduction in sphingomyelin and elevation of ceramide. Pretreatment of cultured neurons with recombinant AHAH1 prevented the cells from undergoing A-beta (Ab)-induced apoptosis.
• Uniprot ID: Q13510
• Molecular Weight: 45 kDa