- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
ASAH1 Antibody (OAPB00726)
| Datasheets/Manuals | Printable datasheet for ASAH1 Antibody (OAPB00726) (OAPB00726) |
|---|
| Predicted Species Reactivity | Bovine|Human|Mouse|Rat |
|---|---|
| Product Format | Liquid. ASAH1 Antibody is supplied in PBS containing 0.02% sodium azide. |
| Clonality | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Conjugation | Unconjugated |
| Application | Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry-Paraffin|Western blot |
| Additional Information | Positive Control: Human heart tissue |
| Reconstitution and Storage | Can be stored at 4°C for three months and -20°C stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Immunogen | ASAH1 antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of the human ASAH1. The immunogen is located within amino acids 240 - 290 of ASAH1. |
| Purification | Affinity Purified |
| Predicted Homology Based on Immunogen Sequence | Bovine (88%) |
| Concentration | 1 mg/ml |
| Specificity | Multiple isoforms of ASAH1 are known to exist. |
| Application Info | WB: 1-2 ug/mL IHC-P: 2.5 ug/mL IF: 20 ug/mL Antibody validated: Western Blot in human samples; Immunohistochemistry in human and rat samples and Immunofluorescence in human and rat samples. All other applications and species not yet tested. Optimal dilutions should be determined by the end user. |
| Publications | Increased placental mitochondrial fusion in gestational diabetes mellitus: an adaptive mechanism to optimize feto-placental metabolic homeostasis?. BMJ Open Diabetes Res Care. 8, (2020). 32144130 |
| Description |
| Gene Symbol | ASAH1 |
|---|---|
| Gene Full Name | N-acylsphingosine amidohydrolase 1 |
| Alias Symbols | AC;ACDase;acid CDase;acid ceramidase;acylsphingosine deacylase;ASAH;N-acylethanolamine hydrolase ASAH1;N-acylsphingosine amidohydrolase;N-acylsphingosine amidohydrolase (acid ceramidase) 1;PHP;PHP32;putative 32 kDa heart protein;SMAPME. |
| NCBI Gene Id | 427 |
| Protein Name | Acid ceramidase|N-acylsphingosine amidohydrolase (acid ceramidase) 1, isoform CRA_c [Homo sapiens] |
| Description of Target | Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid. |
| Uniprot ID | Q13510 |
| Protein Accession # | https://www.ncbi.nlm.nih.gov/protein/EAW63795 |
| Molecular Weight | Predicted: 45 kDa Observed: 49 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
-
What is the species homology for "ASAH1 Antibody (OAPB00726)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Bovine|Human|Mouse|Rat".
-
How long will it take to receive "ASAH1 Antibody (OAPB00726)"?
This item is available "Domestic: within 1-2 weeks delivery | International: 1-2 weeks".
-
What buffer format is "ASAH1 Antibody (OAPB00726)" provided in?
This item is provided in "Liquid. ASAH1 Antibody is supplied in PBS containing 0.02% sodium azide.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "ASAH1 Antibody (OAPB00726)"?
This target may also be called "AC;ACDase;acid CDase;acid ceramidase;acylsphingosine deacylase;ASAH;N-acylethanolamine hydrolase ASAH1;N-acylsphingosine amidohydrolase;N-acylsphingosine amidohydrolase (acid ceramidase) 1;PHP;PHP32;putative 32 kDa heart protein;SMAPME." in publications.
-
What is the shipping cost for "ASAH1 Antibody (OAPB00726)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
-
What is the guarantee for "ASAH1 Antibody (OAPB00726)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
-
Can I get bulk pricing for "ASAH1 Antibody (OAPB00726)"?
You can get bulk pricing for this item by going here.
-
What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "Predicted: 45 kDa Observed: 49 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "ASAH1 Antibody (OAPB00726)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
-
What are positive controls for "ASAH1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
-
What are negative controls for "ASAH1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
-
What other proteins interact with "ASAH1"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
-
What biological processes are associated with "ASAH1"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
-
What cellular components are associated with "ASAH1"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
-
What protein functions are associated with "ASAH1"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.
