ARTS Antibody (OAPB00168)

Catalog No: OAPB00168 (Formerly GWB-1DC0CF)

Size:100UG

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Western blot analysis of ARTS expression in human lung (lane 1), spleen (lane 2), and kidney (lane 3) tissue lysates with ARTS antibody at 2 μg /ml.

Immunohistochemistry of ARTS in human lung tissue with ARTS antibody at 2 μg/mL.

Immunofluorescence of ARTS in Human Lung cells with ARTS antibody at 20 ug/mL.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid. ARTS Antibody is supplied in PBS containing 0.02% sodium azide.
• Clonality: Polyclonal
• Host: Rabbit
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry-Paraffin|Western blot
  Buffer: Antibody is supplied in PBS containing 0.02% sodium azide.
• Reconstitution and Storage: ARTS antibody can be stored at 4C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Immunogen: ARTS antibody was raised against a synthetic peptide corresponding to amino acids near the amino terminus of human ARTS. The immunogen is located within the first 50 amino acids of ARTS.
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Human
• Concentration: 1 mg/ml
• Blocking Peptide: For anti-ARTS antibody is Catalog # OEPB02272
• Application Info: ARTS antibody can be used for detection of ARTS by Western blot at 0.5 to 2 ug/ml. Human lung, kidney and spleen tissue lysates can be used as a positive control and a band at 32 kDa can be detected. (Optimal dilution should be determined by user)
• Reference: Larisch S, Yi Y, Lotan R, Kerner H, et al. A novel mitochondrial septin-like protein, ARTS, mediates apoptosis dependent on its P-loop motif. Nat Cell Biol 2000;2(12):915-21 ;Depraetere V. The ARTS of apoptosis. Nat Cell Biol. 2000;2(12):E219.;Paavola,P., Horelli-Kuitunen,N., Palotie,A. and Peltonen,L. Characterization of a novel gene, PNUTL2, on human chromosome 17q22-q23 and its exclusion as the Meckel syndrome gene. Genomics 1999;55 (1):122-125 ;Zieger,B., Tran,H., Hainmann, I. , Wunderle,D., Zgaga-Griesz,A., Blaser,S. and Ware,J. Characterization and expression analysis of two human septin genes, PNUTL1 and PNUTL2. Gene 2000;261(2):197-203

Target Info

• Gene Symbol: SEPTIN4
• Gene Full Name: septin 4
• Alias Symbols: apoptosis-related protein in the TGF-beta signaling pathway;ARTS;BRADEION;bradeion beta;brain protein H5;C17orf47;CE5B3;CE5B3 beta;cell division control-related protein 2;cerebral protein 7;H5;hCDCREL-2;hucep-7;MART;peanut-like protein 2;PNUTL2;SEP4;SEPT4;septin-4;septin-M;uncharacterized protein C17orf47.
• NCBI Gene Id: 5414
• Protein Name: mitochondrial ARTS protein [Homo sapiens]|Septin-4
• Description of Target: Filament-forming cytoskeletal GTPase (By similarity). May play a role in cytokinesis (Potential). Forms a filamentous structure with SEPT12, SEPT6, SEPT2 and probably SEPT4 at the sperm annulus which is required for the structural integrity and motility of the sperm tail during postmeiotic differentiation (PubMed:25588830). May play a role in platelet secretion. Isoform ARTS, but not the other isoforms, is required for the induction of cell death mediated by TGF-beta and by other apoptotic stimuli.
• Uniprot ID: O43236
• Protein Accession #: https://www.ncbi.nlm.nih.gov/protein/AAG45673
• Molecular Weight: 32 kDa