ARSF ELISA Kit (Human) (OKCD01006)

Catalog No: OKCD01006 (Formerly GWB-KBBAV2)

Size:96WELLS

Human ARSF ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	86-93%	88-95%	86-104%	90-103%
  EDTA plasma(n=5)	80-103%	98-105%	92-99%	80-104%
  heparin plasma(n=5)	87-101%	92-101%	80-89%	80-91%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Arylsulfatase F (ARSF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Arylsulfatase F (ARSF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Arylsulfatase F (ARSF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Arylsulfatase F (ARSF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	91-99	95
  EDTA plasma(n=5)	95-102	98
  heparin plasma(n=5)	97-105	101

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Arylsulfatase F (ARSF) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Arylsulfatase F (ARSF) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-ARSF Microplate: 96 Wells (12 x 8 Well Strips)
  • ARSF Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated ARSF Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids.
• Sensitivity: < 0.112 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Arylsulfatase F (ARSF). No significant cross-reactivity or interference between Arylsulfatase F (ARSF) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: ARSF
• Gene Full Name: arylsulfatase F
• Alias Symbols: arylsulfatase F;ASF.
• NCBI Gene Id: 416
• Protein Name: Arylsulfatase F
• Description of Target: Arylsulfatase F
• Uniprot ID: P54793
• Protein Accession #: NP_001188467
• Nucleotide Accession #: NM_001201538.1
• Protein Size (# AA): 590