ARSA Antibody - C-terminal region (OABB01960)

Catalog No: OABB01960

Size:100UG

Anti-ARSA Picoband antibody, OABB01960, Western blotting
All lanes: Anti ARSA (OABB01960) at 0.5ug/ml
Lane 1: Rat Testis Tissue Lysate at 50ug
Lane 2: Rat Pancreas Tissue Lysate at 50ug
Lane 3: Rat Skeletal Muscle Tissue Lysate at 50ug
Lane 4: Mouse Kidney Tissue Lysate at 50ug
Lane 5: MCF-7 Whole Cell Lysate at 40ug
Predicted bind size: 54KD
Observed bind size: 54KD

Anti-ARSA Picoband antibody, OABB01960, IHC(P)
IHC(P): Human Mammary Cancer Tissue

Anti-ARSA Picoband antibody, OABB01960, IHC(P)
IHC(P): Rat Lung Tissue

Product Info

• Tested Species Reactivity: Human, Mouse, Rat
• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Clonality: Polyclonal
• Clone: Polyclonal
• Isotype: Rabbit IgG
• Host: Rabbit
• Application: Flow cytometry|Immunocytochemistry|Immunofluorescence|Immunohistochemistry|Western blot
• Additional Information: Notes: Tested Species: In-house tested species with positive results.
  By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
  Other applications have not been tested.
  Optimal dilutions should be determined by end users.
  Background: Arylsulfatase A (ARSA) is an enzyme that breaks down sulfatides, namely cerebroside 3-sulfate intocerebroside and sulfate. In humans, arylsulfatase A is encoded by the ARSA gene. ARSA is mapped to 22q13.33. The protein encoded by this gene hydrolyzes cerebroside sulfate to cerebroside and sulfate. Defects in this gene lead to metachromatic leucodystrophy (MLD), a progressive demyelination disease which results in a variety of neurological symptoms and ultimately death. Alternatively spliced transcript variants have been described for this gene.
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Immunogen: Polypeptide
• Purification: Affinity Purified
• Peptide Sequence: Synthetic peptide located within the following region: QALKQLQLLKAQLDAAVTFGPSQVARGED
• Concentration: 500 ug/ml
• Specificity: No cross reactivity with other proteins.
• Application Info: Western blot: 0.1-0.5 ug/ml: Human, Mouse, Rat
  Immunohistochemistry (Paraffin-embedded Section): 0.5-1 ug/ml: Human, Rat: By Heat
  Immunocytochemistry/Immunofluorescence: 2 ug/ml: Human
  Flow Cytometry: 1-3 ug/1x10^6 cells: Human
• Reference: 1. Fujii T; Kobayashi T; Honke K et al. (1992). "Proteolytic processing of human lysosomal arylsulfatase A". Biochim. Biophys. Acta 1122 (1): 93–8.
  2. Kondo R; Wakamatsu N; Yoshino H et al. (1991)."Identification of a mutation in the arylsulfatase A gene of a patient with adult-type metachromatic leukodystrophy". Am. J. Hum. Genet. 48 (5): 971–8.
  3. Matzner U, Herbst E, Hedayati KK, Lüllmann-Rauch R, Wessig C, Schröder S, Eistrup C, Möller C, Fogh J, Gieselmann V (May 2005). "Enzyme replacement improves nervous system pathology and function in a mouse model for metachromatic leukodystrophy". Hum. Mol. Genet. 14 (9): 1139–52.
  
• Storage Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Description: Rabbit IgG polyclonal antibody for Arylsulfatase A(ARSA) detection. Tested with WB, IHC-P, ICC/IF, FCM in Human;Mouse;Rat.

Target Info

• Gene Symbol: ARSA
• Gene Full Name: arylsulfatase A
• Alias Symbols: arylsulfatase A;ASA;cerebroside-sulfatase;epididymis secretory sperm binding protein;MLD.
• NCBI Gene Id: 410
• Protein Name: Arylsulfatase A
• Description of Target: Hydrolyzes cerebroside sulfate.
• Uniprot ID: P15289
• Molecular Weight: 53588 MW