ARPC4 ELISA Kit (Mouse) (OKCD09211)

Catalog No: OKCD09211

Size:96WELLS

ARPC4 ELISA Kit (Mouse) (OKCD09211) Standard Curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Actin Related Protein 2/3 Complex Subunit 4 (ARPC4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Actin Related Protein 2/3 Complex Subunit 4 (ARPC4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Actin Related Protein 2/3 Complex Subunit 4 (ARPC4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Actin Related Protein 2/3 Complex Subunit 4 (ARPC4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Actin Related Protein 2/3 Complex Subunit 4 (ARPC4) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Actin Related Protein 2/3 Complex Subunit 4 (ARPC4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • ARPC4 Microplate: 96 Wells (12 x 8 Well strips)
  • ARPC4 Lyophilized Standard: 2
  • 100X ARPC4 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates and other biological fluids.
• Sensitivity: < 0.065ng/mL

Target Info

• Gene Symbol: Arpc4
• Gene Full Name: actin related protein 2/3 complex, subunit 4
• Alias Symbols: 20kDa;5330419I20Rik;actin-related protein 2/3 complex subunit 4;AI327076;arp2/3 complex 20 kDa subunit;p20-;p20-Arc.
• NCBI Gene Id: 68089
• Protein Name: Actin-related protein 2/3 complex subunit 4
• Description of Target: Functions as actin-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the mother actin filament (By similarity).
• Uniprot ID: P59999
• Protein Accession #: NP_001163956.1
• Nucleotide Accession #: NM_001170485.1