Catalog No: OCOA12467
Size:20UG
Price: $179.00
SKU
OCOA12467
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ARPC2 Protein Lysate (OCOA12467)

Datasheets/ManualsPrintable datasheet for ARPC2 Protein Lysate (OCOA12467)
Product Info
Predicted Species ReactivityHomo sapiens
Product FormatLyophilized
HostHEK293T
Additional InformationComponents: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
::Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution and StorageThe lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
TagC-MYC/DDK
DescriptionARPC2 HEK293T cell transient overexpression lysate (as WB positive control)
Gene SymbolARPC2
Gene Full Nameactin related protein 2/3 complex subunit 2
Alias Symbolsactin related protein 2/3 complex, subunit 2, 34kDa;actin-related protein 2/3 complex subunit 2;ARC34;arp2/3 complex 34 kDa subunit;ARP2/3 protein complex subunit 34;p34-Arc;PNAS-139;PRO2446;testis tissue sperm-binding protein Li 53e.
NCBI Gene Id10109
Protein NameNM_005731, NP_005722, O15144
Description of TargetActin-related protein 2/3 complex subunit 2, actin-related protein 2/3 complex subunit 2 [Homo sapiens], Homo sapiens actin related protein 2/3 complex subunit 2 (ARPC2), transcript variant 2, mRNA
Uniprot IDhttps://www.uniprot.org/uniprot/O15144
Protein Accession #https://www.ncbi.nlm.nih.gov/protein/NP_005722
Nucleotide Accession #https://www.ncbi.nlm.nih.gov/nuccore/NM_005731
Molecular Weight34.3 kDa
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