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Catalog No: OKEH01618
Size:96T
Price: $525.00
SKU
OKEH01618
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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ARNT ELISA Kit (Human) : 96 Wells (OKEH01618)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology ARNT ELISA Kit (Human) (OKEH01618) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ARNT has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ARNT is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ARNT captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 97%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.4% (n = 20)
Mean Inter-assay CV%: < 8.9% (n = 20)
ELISA Kit Component
ComponentAmount
ARNT Microplate96 Wells (12 x 8 Well strips)
ARNT Lyophilized Standard2
100X Biotinylated ARNT Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity0.056 ng/mL
SpecificityNatural and recombinant Human Aryl hydrocarbon receptor nuclear translocator
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolARNT
Gene Full Namearyl hydrocarbon receptor nuclear translocator
Alias Symbolsaryl hydrocarbon receptor nuclear translocator;bHLHe2;class E basic helix-loop-helix protein 2;dioxin receptor, nuclear translocator;HIF1B;HIF1-beta;HIF-1beta;HIF-1-beta;HIF1BETA;hypoxia-inducible factor 1, beta subunit;Hypoxia-inducible factor 1-beta;TANGO.
NCBI Gene Id405
Protein NameP27540
Description of TargetAryl hydrocarbon receptor nuclear translocator
Uniprot IDhttps://www.uniprot.org/uniprot/P27540
Protein Accession #NP_001184254.1
Nucleotide Accession #NM_001197325.1
Protein Size (# AA)789
Molecular Weight87 kDa
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