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Catalog No: OCOA04328
Size:20UG
Price: $275.00
SKU
OCOA04328
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ARL13B Protein Lysate (OCOA04328)

Datasheets/ManualsPrintable datasheet for ARL13B Protein Lysate (OCOA04328)
Product Info
Predicted Species ReactivityHomo sapiens
Product FormatLyophilized
HostHEK293T
Additional InformationComponents: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
::Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution and StorageThe lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
TagC-MYC/DDK
DescriptionARL13B HEK293T cell transient overexpression lysate (as WB positive control)
Gene SymbolARL13B
Gene Full NameADP ribosylation factor like GTPase 13B
Alias SymbolsADP-ribosylation factor-like 13B;ADP-ribosylation factor-like 2-like 1;ADP-ribosylation factor-like protein 13B;ADP-ribosylation factor-like protein 2-like 1;ARL2L1;ARL2-like protein 1;JBTS8.
NCBI Gene Id200894
Protein NameNM_182896, NP_878899, Q3SXY8
Description of TargetADP-ribosylation factor-like protein 13B, ADP-ribosylation factor-like protein 13B isoform 1 [Homo sapiens], Homo sapiens ADP ribosylation factor like GTPase 13B (ARL13B), transcript variant 1, mRNA
Uniprot IDhttps://www.uniprot.org/uniprot/Q3SXY8
Protein Accession #https://www.ncbi.nlm.nih.gov/protein/NP_878899
Nucleotide Accession #https://www.ncbi.nlm.nih.gov/nuccore/NM_182896
Molecular Weight48.5 kDa
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