ARL13B ELISA Kit (Human) (OKEH08582)

Catalog No: OKEH08582

Size:96T

Human ARL13B ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Competitive
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  serum(n=5)	93-101% (n = 20)	107-116% (n = 20)	98-107% (n = 20)	89-98% (n = 20)
  EDTA plasma(n=5)	96-108% (n = 20)	98-106% (n = 20)	88-97% (n = 20)	98-107% (n = 20)
  heparin plasma(n=5)	98-108% (n = 20)	89-99% (n = 20)	105-115% (n = 20)	95-105% (n = 20)

• ELISA Kit Principle: Aviva Systems Biology ARL13B ELISA Kit (OKEH08582) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-ARL13B antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated ARL13B and incubated. The ARL13B found in the sample or standards competes with the biotinylated ARL13B for limited binding sites on the immobilized anti-ARL13B antibody. Excess unbound biotinylated ARL13B and sample or standard ARL13B is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated ARL13B captured in the well and inversely proportional to the amount of ARL13B which was contained in the sample or standard.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 111% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=3.8% (n = 20)
  Mean Inter-assay CV%: <=11.3% (n = 20)
• ELISA Kit Component:
  • Anti-ARL13B Microplate: 96 Wells (12 x 8 Well strips)
  • ARL13B Lyophilized Standard: 2 x 100 ng
  • Sample Diluent: 1 x 20 mL
  • 100X ARL13B-Biotin Complex: 1 x 60 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Biotin Complex Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Cilium-specific protein required to control the microtubule-based, ciliary axoneme structure. May act by maintaining the association between IFT subcomplexes A and B. Binds GTP but is not able to hydrolyze it; the GTPase activity remains unclear. Required to pattern the neural tube. Involved in cerebral cortex development: required for the initial formation of a polarized radial glial scaffold, the first step in the construction of the cerebral cortex, by regulating ciliary signaling. Regulates the migration and placement of postmitotic interneurons in the developing cerebral cortex. May regulate endocytic recycling traffic; however, additional evidences are required to confirm these data.
  Subcellular Location: Cell projection Cilium membrane Lipid-anchor Cell projection Cilium Associates to the cilium membrane via palmitoylation. Localizes to proximal ciliary membranes, to an inversin-like subciliary membrane compartment, excluding the transition zone.
  Unigene: Hs.533086
  SMR: Q3SXY8
  String: 9606.ENSP00000377769
  KEGG: hsa:200894
  MIM: 608922
  Pfam: PF00025
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 0.134ng/mL
• Specificity: Natural and recombinant Human ADP-ribosylation factor-like protein 13B
• Assay Info: Assay Methodology: Quantitative Competitive ELISA

Target Info

• Gene Symbol: ARL13B
• Gene Full Name: ADP ribosylation factor like GTPase 13B
• Alias Symbols: ADP-ribosylation factor-like 13B;ADP-ribosylation factor-like 2-like 1;ADP-ribosylation factor-like protein 13B;ADP-ribosylation factor-like protein 2-like 1;ARL2L1;ARL2-like protein 1;JBTS8.
• NCBI Gene Id: 200894
• Protein Name: Q3SXY8
• Description of Target: ADP-ribosylation factor-like protein 13B
• Uniprot ID: https://www.uniprot.org/uniprot/Q3SXY8
• Protein Accession #: NP_001167621.1
• Nucleotide Accession #: NM_001174150.1