Catalog No: OKCD09226
Size:96WELLS
Price: $850.00
SKU
OKCD09226
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
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Datasheets/Manuals | Printable datasheet for ARHGEF7 ELISA Kit (Rat) (OKCD09226) |
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Predicted Species Reactivity | Rat|Rattus norvegicus | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric | ||||||||||||||||||||||
ELISA Kit Duration | 3h | ||||||||||||||||||||||
ELISA Kit Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rho Guanine Nucleotide Exchange Factor 7 (ARHGEF7). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Rho Guanine Nucleotide Exchange Factor 7 (ARHGEF7). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rho Guanine Nucleotide Exchange Factor 7 (ARHGEF7), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Rho Guanine Nucleotide Exchange Factor 7 (ARHGEF7) in the samples is then determined by comparing the O.D. of the samples to the standard curve. | ||||||||||||||||||||||
ELISA Kit Range | 0.156-10ng/mL | ||||||||||||||||||||||
ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Rho Guanine Nucleotide Exchange Factor 7 (ARHGEF7) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Rho Guanine Nucleotide Exchange Factor 7 (ARHGEF7) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||||
Sample Type | Tissue homogenates and other biological fluids. | ||||||||||||||||||||||
Sensitivity | < 0.064ng/mL |
Gene Symbol | Arhgef7 |
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Gene Full Name | Rho guanine nucleotide exchange factor 7 |
Alias Symbols | beta-Pix;P85spr;Pak3bp;PAK-interacting exchange factor beta;Rho guanine nucleotide exchange factor (GEF) 7;rho guanine nucleotide exchange factor 7. |
NCBI Gene Id | 114559 |
Protein Name | Rho guanine nucleotide exchange factor 7 |
Description of Target | Acts as a RAC1 guanine nucleotide exchange factor (GEF) and can induce membrane ruffling. Functions in cell migration, attachment and cell spreading. Promotes targeting of RAC1 to focal adhesions. May function as a positive regulator of apoptosis. Downstream of NMDA receptors and CaMKK-CaMK1 signaling cascade, promotes the formation of spines and synapses in hippocampal neurons (By similarity). |
Uniprot ID | O55043 |
Protein Accession # | NP_001106994.1 |
Nucleotide Accession # | NM_001113522.1 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
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