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Datasheets/Manuals | Printable datasheet for ARHGEF5 Antibody (OAAF04193) |
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Predicted Species Reactivity | Human|Mouse|Rat |
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Product Format | Liquid |
Clonality | Polyclonal |
Host | Rabbit |
Application | Enzyme-linked immunosorbent assay|Western blot |
Reconstitution and Storage | -20°C |
Immunogen | The antiserum was produced against synthesized peptide derived from human ARHGEF5. |
Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Specificity | ARHGEF5 antibody detects endogenous levels of total ARHGEF5 protein. |
Formulation | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Dilution | The optimal working dilution should be determined by the end user. |
Application Info | WB: 1:500~1:3000; ELISA: 1:20000 |
Reference | Chan A.M.-L., Oncogene 9:1057-1063(1994). Jikuya H., Submitted (APR-2004) to the EMBL/GenBank/DDBJ databases. Hillier L.W., Nature 424:157-164(2003) |
Gene Symbol | ARHGEF5 |
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Gene Full Name | Rho guanine nucleotide exchange factor 5 |
Alias Symbols | ephexin3;ephexin-3;GEF5;guanine nucleotide regulatory protein TIM;oncogene TIM;P60;p60 TIM;Rho guanine nucleotide exchange factor (GEF) 5;rho guanine nucleotide exchange factor 5;TIM;TIM1;transforming immortalized mammary oncogene. |
NCBI Gene Id | 7984 |
Protein Name | Rho guanine nucleotide exchange factor 5 |
Description of Target | Guanine nucleotide exchange factor which activates Rho GTPases (PubMed:15601624). Strongly activates RHOA (PubMed:15601624). Also strongly activates RHOB, weakly activates RHOC and RHOG and shows no effect on RHOD, RHOV, RHOQ or RAC1 (By similarity). Involved in regulation of cell shape and actin cytoskeletal organization (PubMed:15601624). Plays a role in actin organization by generating a loss of actin stress fibers and the formation of membrane ruffles and filopodia (PubMed:14662653). Required for SRC-induced podosome formation (By similarity). Involved in positive regulation of immature dendritic cell migration (By similarity). |
Uniprot ID | Q12774 |
Protein Accession # | NP_005426.2 |
Molecular Weight | 60 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "ARHGEF5 Antibody (OAAF04193)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human|Mouse|Rat".
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How long will it take to receive "ARHGEF5 Antibody (OAAF04193)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".
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What buffer format is "ARHGEF5 Antibody (OAAF04193)" provided in?
This item is provided in "Liquid".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "ARHGEF5 Antibody (OAAF04193)"?
This target may also be called "ephexin3;ephexin-3;GEF5;guanine nucleotide regulatory protein TIM;oncogene TIM;P60;p60 TIM;Rho guanine nucleotide exchange factor (GEF) 5;rho guanine nucleotide exchange factor 5;TIM;TIM1;transforming immortalized mammary oncogene." in publications.
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What is the shipping cost for "ARHGEF5 Antibody (OAAF04193)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "ARHGEF5 Antibody (OAAF04193)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "ARHGEF5 Antibody (OAAF04193)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "60 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "ARHGEF5 Antibody (OAAF04193)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "ARHGEF5"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "ARHGEF5"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "ARHGEF5"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "ARHGEF5"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "ARHGEF5"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "ARHGEF5"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.