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Datasheets/Manuals | Printable datasheet for OAAB14205 |
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Predicted Species Reactivity | Human |
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Product Format | Liquid. PBS with 0.09% (W/V) sodium azide. |
Clonality | Polyclonal |
Isotype | Ig |
Host | Rabbit |
Application | WB |
:: | Function: Acts as guanine nucleotide exchange factor (GEF) for RHOA, RAC1 and CDC42 GTPases. Binding of APC may activate RAC1 GEF activity. The APC-ARHGEF4 complex seems to be involved in cell migration as well as in E-cadherin-mediated cell-cell adhesion Required for MMP9 up-regulation via the JNK signaling pathway in colorectal tumor cells. Involved in tumor angiogenesis and may play a role in intestinal adenoma formation and tumor progression. Cellular Location: Isoform 3: Cytoplasm. Cell projection, ruffle membrane; Peripheral membrane protein; Cytoplasmic side (Probable). Note=Associated with membrane ruffles (Probable) Tissue Location: Expressed at high levels in the brain, skeletal muscle and testis and at low levels in the kidney, lung, small intestine, ovary and prostate. Expression is aberrantly enhanced in most colorectal tumors. |
Reconstitution and Storage | Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles. |
Immunogen | This ARHGEF4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 573-599 amino acids from the C-terminal region of human ARHGEF4. |
Purification | This antibody is purified through a protein A column, followed by peptide affinity purification. |
Predicted Homology Based on Immunogen Sequence | Human |
Concentration | Approximately 0.5mg/ml. Actual concentration varies with each lot. |
Application Info | WB: 1:1000 |
Gene Symbol | ARHGEF4 |
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Alias Symbols | ASEF, GEF4, STM6, ASEF1, SMIM39 |
NCBI Gene Id | 50649 |
Protein Name | Rho guanine nucleotide exchange factor 4 |
Description of Target | Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form complex with G proteins and stimulate Rho-dependent signals. This protein is similar to rat collybistin protein. Alternative splicing of this gene generates two transcript variants which encode different isoforms. Also there is possibility for the usage of multiple polyadenylation sites for this gene. |
Uniprot ID | Q9NR80 |
Protein Accession # | NP_056135.2 |
Molecular Weight | 79067 Da |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "ARHGEF4 Antibody - C-terminal region (OAAB14205)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human".
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How long will it take to receive "ARHGEF4 Antibody - C-terminal region (OAAB14205)"?
This item is available "Domestic: within 1-2 week delivery | International: within 1-2 week delivery".
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What buffer format is "ARHGEF4 Antibody - C-terminal region (OAAB14205)" provided in?
This item is provided in "Liquid. PBS with 0.09% (W/V) sodium azide. ".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "ARHGEF4 Antibody - C-terminal region (OAAB14205)"?
This target may also be called "ASEF, GEF4, STM6, ASEF1, SMIM39" in publications.
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What is the shipping cost for "ARHGEF4 Antibody - C-terminal region (OAAB14205)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "ARHGEF4 Antibody - C-terminal region (OAAB14205)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "ARHGEF4 Antibody - C-terminal region (OAAB14205)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "79067 Da".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "ARHGEF4 Antibody - C-terminal region (OAAB14205)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "ARHGEF4"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "ARHGEF4"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "ARHGEF4"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "ARHGEF4"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "ARHGEF4"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "ARHGEF4"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.